Inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, fibroblasts and application

Abstract

The invention provides an inducing medium and method for inducing fibroblasts to secrete antimicrobial peptide, the fibroblasts and application, and relates to the field of cell engineering. The inducing medium contains a basic medium, platelet lysate and a TGF-beta signal channel inhibitor. The inducing medium can activate the adipose differentiation proficiency of the skin fibroblasts and secrete the antimicrobial peptide in the adipose differentiation process. When the inducing medium is used for inducing the fibroblasts to secrete the antimicrobial peptide, operation is easy, the controllability is high, and the problem of lacking the fibroblasts capable of secreting the antimicrobial peptide in vitro in the prior art is solved.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to an induction medium and method for inducing fibroblasts to secrete antimicrobial peptides, fibroblasts and applications. Background technique [0002] Human skin fibroblasts, located in the dermis of the skin, are a kind of cells with certain differentiation potential originating from the mesenchyme. At present, it is believed that skin fibroblasts have the function of secreting collagen, elastic fibers and other extracellular matrix, so they have a wide range of applications in the fields of medical plastic surgery and skin damage repair. [0003] The latest research shows that skin fibroblasts have the potential to differentiate into fat in the dermis of the skin, and can secrete antimicrobial peptides during the differentiation process, which is crucial to the innate immune function of the skin. Antimicrobial peptide is a new type of antibacterial protein, which has many advan...

AI Technical Summary

Problems solved by technology

Antibiotics and hormonal drugs have the disadvantages of drug resistance, strong side effects, and large individual differences.

These two types of treatment methods all have the disadvantages of long treatment cycle, short validity period, and inability to cure the disease. Especially when antibiotics are used for treatment, with the increase in the use of antibiotics, the emergence of various drug-resistant bacteria makes the effect of antibiotic treatment more and more effective. Difference

The antimicrobial peptide itself is a biological macromolecule. If it is applied externally, it cannot be absorbed through the skin. Oral administration will lead to the degradation of the antimicrobial peptide.

Directly transplanted dermal fibroblasts cannot secrete antimicrobial peptides and have no effect on alleviating inflammatory skin problems

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