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Method for improving fish fertility

A fecundity, fish technology, applied in the field of transgenic, can solve the problem of increasing the fecundity of zebrafish, not raised

Inactive Publication Date: 2019-08-23
HUNAN UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Zebrafish is also a relatively easy fish to raise, but there is no way to increase the fecundity of zebrafish

Method used

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Embodiment Construction

[0049] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0050] The application principle of the present invention will be further described below in conjunction with accompanying drawings and specific embodiments:

[0051] A method for increasing fish fecundity, comprising the steps of:

[0052] (1) Cloning of fish leptin gene and construction of its transgenic vector:

[0053] S100, referring to the zebrafish mRNA sequence (NM_001128576.1) from the National Center for Biotechnology Information NCBI, cloned the zebrafish leptin gene, and designed a pair of specific primers to introduce restriction sites:

[0054] leptin-HindIII-F: 5'-CTCAAGCTTATGCGTTTTCCAGCTCTCCG 3';

[0055]...

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Abstract

The invention belongs to the technical field of transgenosis, and discloses a method for improving the fish fertility. The method comprises the following steps of 1, cloning a fish leptin gene and constructing a transgenosis vector thereof; 2, before micro-injection, performing transgenosis vector processing, wherein de-toxicity treatment and anti-cascading treatment are included; 3, performing in-vitro synthesis of Tol2 transposase mRNA; 4, performing micro-injection on a plasmid DNA obtained after transgenosis vector processing and the Tol2 transposase mRNA on zebra fish embryos; 5, adoptingthe zebra fish embryos obtained after micro-injection as a generation P0, and after the generation P0 is cultured to be sexually mature, performing test mating with wild female zebra fish to obtain alater generation F1, and after male individuals of the generation F1 is cultivated to be sexually mature, adopting selfing to obtain a homozygous generation F2. The purpose of improving the zebra fish fertility is achieved.

Description

technical field [0001] The invention belongs to the field of transgenic technology, in particular to a method for increasing fish fecundity. Background technique [0002] my country is a big aquaculture country. For a long time, my country's aquaculture production has accounted for two-thirds of the world's total production. At present, my country is rapidly moving towards a strong aquaculture country. There are many types of aquaculture in my country, including more than 200 species of fish, shrimp, shellfish, and algae. At present, the development of improved aquaculture species in my country is mainly based on traditional breeding techniques such as selection, hybridization, and chromosome engineering. New The speed of species development is slow. At present, the coverage rate of aquatic species in my country is only about 20%. Fish-based aquatic species have strong fecundity and physiological characteristics such as in vitro fertilization and in vitro hatching are very c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/85A01K67/027
CPCC07K14/461C12N15/85A01K67/0278A01K2227/40A01K2267/02A01K2217/05
Inventor 张运生杨品红刘良国
Owner HUNAN UNIV OF ARTS & SCI