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Microorganism evaluation method for ultraviolet barrier property of anti-ultraviolet product

A technology of protection performance and evaluation method, which is applied in the field of microbial evaluation of ultraviolet protection performance, can solve the problems of low measurement accuracy, short experimental period, cost, unfavorable repeated tests, etc., to achieve simple detection process, avoid health damage, and high repeatability Effect

Pending Publication Date: 2019-08-23
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the patent CN108956606A discloses a detection method for sunscreen efficacy of sunscreen skin care products; this technology uses zebrafish as the experimental carrier, and evaluates the sunscreen efficacy of sunscreen skin care products by comparing the integrity of zebrafish fins before and after ultraviolet irradiation. Short and low cost; but this technical solution needs to dissolve the sunscreen product in the breeding water, the measurement range is wide, it is not conducive to repeated tests, and the measurement accuracy is low

Method used

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  • Microorganism evaluation method for ultraviolet barrier property of anti-ultraviolet product

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Embodiment 1

[0026] The invention discloses a microbial evaluation method for the ultraviolet protection performance of an anti-ultraviolet product, comprising the following steps:

[0027] (1) Pretreatment of bacterial strains: select the Staphylococcus aureus bacterial liquid in the stable phase after being inoculated in the broth medium shaker for 17 hours, mix and shake 80% bacterial liquid with 20% glycerin to obtain the mixed bacterial liquid, respectively Pour it into several EP tubes, mix and shake well, and put it in a refrigerator at minus 18°C ​​until use;

[0028] (2) Pretreatment of anti-ultraviolet products: select the sunscreen of a certain brand of SPF20 as a standard sample, and apply the sunscreen on 9 special UV-transparent glasses made by JGS2 with a diameter of 10cm at an amount of 2 mg per square centimeter;

[0029] (3) Coating of bacteria solution: take out a tube of the mixed bacteria solution obtained in step (1) and dilute 10 5 After multiplying, it is spread on...

Embodiment 2

[0033] The invention discloses a microbial evaluation method for the ultraviolet protection performance of an anti-ultraviolet product, comprising the following steps:

[0034] (1) Pretreatment of bacterial strains: select the Staphylococcus aureus bacterial liquid in the stable phase after being inoculated in the broth medium shaker for 17 hours, mix and shake 80% bacterial liquid with 20% glycerin to obtain the mixed bacterial liquid, respectively Pour it into several EP tubes, mix and shake well, and put it in a refrigerator at minus 18°C ​​until use;

[0035] (2) Pretreatment of anti-ultraviolet products: select the sunscreen of a certain brand of SPF30 as a standard sample, and apply the sunscreen on 9 special UV-transmitting glasses made by JGS2 with a diameter of 10cm at an amount of 2 mg per square centimeter;

[0036] (3) Coating of bacteria solution: take out a tube of the mixed bacteria solution obtained in step (1) and dilute 10 5 After multiplying, spread on 12 a...

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Abstract

The invention discloses a microorganism evaluation method for the ultraviolet barrier property of an anti-ultraviolet product. The method comprises the following steps of (1) selecting a bacterial liquid in a stable period, shaking the bacterial liquid and glycerol for uniform mixing, pouring the mixture into a test tube, and placing the test tube in a refrigerator for refrigeration for later use;(2) coating ultraviolet-permeable glass with the anti-ultraviolet product; (3) after the mixed bacterial liquid obtained in step (1) is diluted, coating an agar plate with the mixed bacterial liquid,and setting sampling points; (4) covering the agar plate obtained in step (3) with the glass obtained in step (2) and irradiating the agar plate under an ultraviolet lamp; (5) culturing strains subjected to the ultraviolet irradiation treatment in step (4), and carrying out colony counting. According to the method, ultraviolet rays are used for irradiating bacteria protected by sunscreen creams,the growth conditions of the bacteria irradiated in different lengths of time under the protection of different types of sunscreen creams are observed, the protection effect of the sunscreen creams onthe bacteria is judged, a mathematical model is established and statistically correlated with existing standards (SPF) to replace an original experimental method, the detection steps are simplified,and the detection cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a microbial evaluation method for the ultraviolet protection performance of anti-ultraviolet products. Background technique [0002] With the continuous improvement of people's material living standards and the change of life concept, the skin will be exposed to ultraviolet radiation for a long time when going out to work, travel, shopping and other activities; Some ways to block or absorb UV rays. General sun protection methods: apply sun protection products, use sun protection clothing and other items, eat sun protection food, etc.; due to the simplicity and convenience of applying sun protection skin care products, more and more people will apply sun protection skin care products when going out, and the sun protection products on the market There are many types of products. Therefore, how to effectively evaluate the efficacy of a sunscreen product and facilitate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12Q1/06C12R1/445
CPCC12Q1/14C12Q1/06G01N2333/31
Inventor 林元杰张志坤任宁君袁港龙朝东
Owner SOUTHWEST MEDICAL UNIVERISTY