Preparation method of in vivo targeted ovarian cancer cell imaging nano material composed of multifunctional macromolecule and metal-organic frames (MOFs)

A technology of metal-organic framework and ovarian cancer cells, which is applied in the field of nano-biomedical materials, can solve the problems of poor targeting, and achieve the effect of satisfying production and application, good physical and chemical stability, and simple process

Inactive Publication Date: 2019-08-27
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the traditional MOF preparation research, biological imaging mainly includes: nanoparticles, such as Chinese invention patent publication number: CN107349964A A kind of nanoparticle @ small size metal organic framework material preparation method and rare earth oxyfluoride nanomaterials, Chinese invention patent publication number : CN105505392A rare earth oxyfluoride nanomaterial and its preparation method and application, although these MOFs have better biocompatibility, but poor targeting

Method used

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  • Preparation method of in vivo targeted ovarian cancer cell imaging nano material composed of multifunctional macromolecule and metal-organic frames (MOFs)
  • Preparation method of in vivo targeted ovarian cancer cell imaging nano material composed of multifunctional macromolecule and metal-organic frames (MOFs)
  • Preparation method of in vivo targeted ovarian cancer cell imaging nano material composed of multifunctional macromolecule and metal-organic frames (MOFs)

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Embodiment 1

[0028]A multifunctional polymer and metal-organic framework in vivo targeting ovarian cancer cell imaging nanomaterials, synthesized by RAFT reaction with polymers targeting ovarian cancer cell peptides, and then coordinated with Eu-MOF synthesized by solvothermal method , to obtain multifunctional nanomaterials with targeting ovarian cancer cells and in vivo fluorescence imaging, the steps are as follows:

[0029] (1) Preparation of rare earth metal europium-organic framework (Eu-MOF) nanoparticles: the same amount of 0.001 mol of H 2 IPA and Eu(NO 3 ) 3 ·6H 2 O was dissolved in a mixed solution of 6 mL of dimethylformamide (DMF) and 24 mL of water, and 0.6 g of polyvinylpyrrolidone-K30 (PVP, K30) was added as a surfactant. Stir and heat in the bath for 10 min, filter and dry under vacuum at room temperature to obtain the product Eu-MOF nanoparticles;

[0030] (2) Synthesis of high-molecular polymers grafted with targeting peptides: 0.01 mol NIPAM, 0.1 mmol trithiocarbona...

Embodiment 2

[0037] A nanomaterial for in vivo targeting ovarian cancer cell imaging composed of multifunctional polymers and metal-organic frameworks, according to the following steps:

[0038] (1) Preparation of rare earth metal europium organic framework (Eu-MOF) nanoparticles: 0.001 mol of H 2 IPA and 0.002 molEu(NO 3 ) 3 ·6H 2 O was dissolved in a mixed solution of 6 mL of dimethylformamide (DMF) and 24 mL of water, and 0.6 g of polyvinylpyrrolidone-K30 (PVP, K30) was added as a surfactant. Stir and heat in the bath for 10 min, filter and dry under vacuum at room temperature to obtain the product Eu-MOF nanoparticles.

[0039] (2) Synthesis of polymers grafted with targeted peptides: 0.01 mol NIPAM, 0.1 mmol trithiocarbonate (DATC), 0.4 mmol azobisisobutyronitrile (AIBN) were dissolved in 4 In mL of anhydrous dioxane, in which DATC is used as a chain transfer agent and AIBN is used as an initiator, the reactant is stirred and heated in an oil bath at 65°C for 24 h, and the product...

Embodiment 3

[0043] A nanomaterial for in vivo targeting ovarian cancer cell imaging composed of multifunctional polymers and metal-organic frameworks, according to the following steps:

[0044] (1) Preparation of rare earth metal europium organic framework (Eu-MOF) nanoparticles: 0.001 mol of H 2 IPA and 0.005 molEu(NO 3 ) 3 ·6H 2 O was dissolved in a mixed solution of 6 mL dimethylformamide (DMF) and 24 mL water, and 0.6 g of polyvinylpyrrolidone-K30 (PVP, K30) was added as a surfactant. The reactants were stirred and heated in an oil bath at 100 °C for 10 min, filtered and then vacuum-dried at room temperature to obtain the product Eu-MOF nanoparticles.

[0045] (2) Synthesis of polymers grafted with targeted peptides: 0.01 mol NIPAM, 0.1 mmol trithiocarbonate (DATC), 0.4 mmol azobisisobutyronitrile (AIBN) were dissolved in 4 mL of anhydrous dioxane with DATC as chain transfer agent and AIBN as initiator. The reactant was stirred and heated in an oil bath at 65°C for 24 h, the prod...

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Abstract

The invention relates to a preparation method of in vivo targeted ovarian cancer cell imaging nano material composed of multifunctional macromolecule and metal-organic frames (MOFs). A polymer with atargeted ovarian cancer cell polypeptide is synthesized through an RAFT reaction, and the polymer is coordinated with Eu-MOF synthesized through a solvent thermal method, so that nano particles with targeted ovarian cancer cells and an in vivo fluorescent imaging function are obtained. The fluorescent material obtained through the method has the characteristics of good biocompatibility, strong targeted performance and strong illuminating intensity. The targeted polypeptide is grated onto the polymer through the RAFT reaction, then the polymer is constructed into rare earth MOF nano particles through a coordination method, so that the MOF nano particles have both fluorescent performance and a targeting function. The preparation method of the invention is simple in technique and strong in operability, and can further meet demands of production and application.

Description

technical field [0001] The invention relates to a preparation method of an in vivo targeting ovarian cancer cell imaging nanomaterial composed of multifunctional polymers and metal organic frameworks (MOFs). The invention belongs to the field of nano biomedical materials. Background technique [0002] In recent years, the synthesis of nano-rare-earth metal-organic frameworks (MOFs) materials and their applications in biomedicine, especially in tumor diagnosis and treatment, have attracted widespread attention from scientists. Known for their porosity, MOFs have been widely used in various fields, such as nonlinear optics, gas adsorption, catalysis, and chemical sensing. Due to the unique optical properties of nano-rare earth MOFs, which can be used in biological imaging, such as fluorescence imaging, the research on nano-rare earth MOFs provides a new platform for the development of new diagnostic and therapeutic integration reagents. Nevertheless, MOFs still have certain ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0019A61K49/0054A61K49/0056A61K49/0093
Inventor 何丹农王杰林于建树王萍金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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