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Identification method for biological function component of marine hypoglycemic health food

A technology for health food and functional ingredients, which is applied in material separation, analysis materials, measuring devices, etc., can solve the problems of synthetic astaxanthin not having the effect of natural ingredients, high price of natural astaxanthin, and restrictions on large-scale production and application. , to achieve significant free radical scavenging effect, the effect of preventing kidney damage

Inactive Publication Date: 2019-08-30
JIANGSU ZODIAC MARINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of natural astaxanthin is relatively high, and synthetic astaxanthin does not have the efficacy of its natural ingredients, which limits its large-scale production and application in the industry

Method used

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  • Identification method for biological function component of marine hypoglycemic health food
  • Identification method for biological function component of marine hypoglycemic health food
  • Identification method for biological function component of marine hypoglycemic health food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Acquisition of astaxanthin high-yield and expression-promoting genes

[0019] The marine red yeast (Rhodotorula benthica) strain was purchased from the China General Microorganism Culture Collection and Management Center, with a preservation number of CGMCC2.5690. Previous studies have proved that the strain has a high astaxanthin production, but it grows in a high-pressure, high-salt environment in the ocean. It is not easy to be cultivated in large-scale industrialization, and cannot be directly applied to the production of industrialized astaxanthin. The above-mentioned problems have not been solved. The present invention explores its molecular mechanism by excavating the high-yield astaxanthin-promoting gene of the marine Rhodotorula yeast, and extracts its genomic DNA for whole-genome sequencing. The genome sequencing was commissioned by Nanjing GenScript Company through the SOLEXA sequencing platform, and the Escherichia coli expression system (E.coli DH...

Embodiment 2

[0021] Example 2: Astaxanthin high-yield expression-promoting gene transfer into cloning vector

[0022] The nucleotide sequences of XQS1, XQS2, XQS3, XQS4, XQS5, XQS6, XQS7, XQS8, XQS9, and XQS10 were synthesized by Nanjing KingScript Biotechnology Co., Ltd., and the 5' end of the synthesized sequence was also connected with an NcoI restriction site point, the 3' end is also connected with a SwaI restriction site. The synthetic XQS1, XQS2, XQS3, XQS4, XQS5, XQS6, XQS7, XQS8, XQS9, and XQS10 nucleotide sequences were respectively linked into the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were as follows: According to Promega’s product instructions, the recombinant cloning vectors pGEM-XQS01, pGEM-XQS02, pGEM-XQS03, pGEM-XQS04, pGEM-XQS05, pGEM-XQS06, pGEM-XQS07, pGEM-XQS08, pGEM-XQS09, pGEM-XQS10, The vector structure includes: Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ represen...

Embodiment 3

[0023] Embodiment 3: the preparation of the protoplast of Pichia pastoris

[0024] The protoplasts of Pichia pastoris (Pichia pastoris) were prepared, and the strains of Pichia pastoris (Pichia pastoris) were purchased from the China General Microorganism Culture Collection and Management Center (preservation number CGMCC1.2.5688), and the source of the strain was Fuzhou University Life Science and School of Engineering, the preparation process is as follows: Dilute Pichia pastoris somatic cell culture solution to 1×10 6 cells / ml, add 15ml of EDTA (0.05mol / L) and 1ml of B-mercaptoethanol (015mol / L) to 5ml of diluent, pretreat at 35°C for 15min, then centrifuge at 3500r / min for 10min to collect the bacteria The body was washed twice with hypertonic buffer, then 5ml of 1% cellulase was added to mix well, and then placed in a water bath at 30°C for 60 minutes, then centrifuged at 3500r / min for 10 minutes in a low-temperature centrifuge to collect the cells, and then used a high ...

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Abstract

The invention provides an identification method for a biological function component of a marine hypoglycemic health food, specifically, the method identifies major components in the marine hypoglycemic health food by employing a high-performance liquid chromatography diode array detector, obtains a fingerprint spectrum with a good degree of separation and a good peak shape by optimizing a series of chromatographic conditions, and identifies the biological function component of the marine hypoglycemic health food by comparison with a standard product.

Description

technical field [0001] The invention relates to the field of health food, in particular to a method for identifying biological functional components of marine hypoglycemic health food. Background technique [0002] Health food refers to non-toxic and harmless food for human consumption that is suitable for specific groups of people, can regulate body functions, prevent diseases, improve health or help the body recover, but not for the purpose of treatment, and meets the nutritional requirements. . Marine health food is a category of health food, which refers to the health food that uses pure marine health food, functional factors or marine health food and functional factors as the main ingredients. It is a kind of health food favored by researchers and consumers in recent years. [0003] Astaxanthin, also known as astaxanthin, is a red carotenoid found in shrimp and crab shells, oysters, salmon, algae, and fungi. In recent years, studies have shown that natural astaxanthi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 聂毅宗有田
Owner JIANGSU ZODIAC MARINE BIOTECH
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