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Harengula zunasi glycoprotein extracting method and application

An extraction method, the technology of meadow carp, is applied in the field of biochemistry to achieve the effects of ensuring activity, increasing extraction rate, and improving purity

Active Publication Date: 2019-09-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no studies and reports on the extraction method of medlar glycoprotein and its regulatory effect on the immune system

Method used

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  • Harengula zunasi glycoprotein extracting method and application
  • Harengula zunasi glycoprotein extracting method and application
  • Harengula zunasi glycoprotein extracting method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting medlar glycoprotein, comprising the following steps:

[0037] ①Pretreatment: Clean the scales of the fresh medaka, drain the water, cut the fish meat into pieces, and grind them in a meat grinder to obtain the medaka puree, which is weighed;

[0038] ②Ultrasonic treatment + hot water leaching: Mix the medlar meat paste obtained in step ① with distilled water at a material-to-liquid ratio of 1:20 (g / mL), and perform ultrasonic treatment for 30 minutes to obtain the medlar mixture and put it in a water bath. Extract in hot water at 100°C for 3 hours, filter, and use the obtained filtrate for later use. Mix the obtained filter residue with distilled water at a solid-liquid ratio of 1:20 (g / mL) and ultrasonically treat it for 30 minutes, then extract in hot water at 100°C for 1 hour, and filter , combining the filtrates twice to obtain the medlar glycoprotein mixed solution;

[0039] ③ Concentrated alcohol precipitation: Add the mixed solution obtai...

Embodiment 2

[0045] The operation steps of embodiment 2 and embodiment 1 are basically the same, and its difference is:

[0046] In the step ② ultrasonic treatment + hot water leaching: mix the medlar meat paste obtained in step ① with distilled water at a material-to-liquid ratio of 1:30 (g / mL), and ultrasonically treat it at 25°C for 40 minutes to obtain the medlar The mixture was placed in a water bath, leached with hot water at 90°C for 2 hours, filtered, and the obtained filtrate was used for later use. The obtained filter residue was mixed with distilled water at a material-liquid ratio of 1:30 (g / mL), then ultrasonically treated for 40 minutes, and then heated at 90°C Water leaching for 1 hour, filtering, and combining the two filtrates to obtain a medlar glycoprotein mixed solution;

[0047] Step ③ concentrated alcohol precipitation: put the mixed solution obtained in step ② into a rotary evaporator, evaporate and concentrate at 60°C to 1 / 5 of the original volume, add 3 times the v...

Embodiment 3

[0050] The operation steps of embodiment 3 and embodiment 1 are basically the same, and its difference is:

[0051] In the step ② ultrasonic treatment + hot water leaching: mix the medlar meat paste obtained in step ① with distilled water at a material-to-liquid ratio of 1:40 (g / mL), and ultrasonically treat it for 20 minutes at 30°C to obtain the medaka The fish mixture was placed in a water bath, leached in hot water at 80°C for 4 hours, filtered, and the obtained filtrate was used for later use. The obtained filter residue was mixed with distilled water at a solid-liquid ratio of 1:40 (g / mL), then ultrasonically treated for 20 minutes, and then heated at 80°C Hot water leaching for 1 hour, filtering, and combining the two filtrates to obtain a mixed solution of medlar glycoprotein;

[0052] Step ③ concentrated alcohol precipitation: put the mixed solution obtained in step ② into a rotary evaporator, evaporate and concentrate at 70°C to 1 / 5 of the original volume, add 3 time...

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Abstract

The invention relates to the technical field of biochemistry, and discloses a harengula zunasi glycoprotein extracting method and application. The extracting method comprises steps of pretreatment, ultrasonic treatment plus hot water extraction, concentrated alcohol precipitation, washing and purification, dialysis and freeze drying and the like. Through a single factor experiment and an orthogonal experiment method, the harengula zunasi glycoprotein extracting process is comparatively optimized, and the best harengula zunasi glycoprotein extracting process parameters are obtained. The harengula zunasi glycoprotein extracting method disclosed in the invention has the advantages that the design is reasonable, the process is simple, the product extraction rate is high, the use of a large amount of a toxic solvent is not needed, the product safety is good, the preparation cost is low, promotion and application are easy and the like. A cell experiment verifies and shows that the harengulazunasi glycoprotein plays a significant role in regulation of cellular immunity, which provides a reference for application of the harengula zunasi glycoprotein to the development of pharmaceuticals and foods.

Description

technical field [0001] The invention relates to the technical field of biochemistry, and more specifically, to a method for extracting medaka glycoprotein and its application. Background technique [0002] Glycoprotein is a substance in which oligosaccharide chains and polypeptides are covalently linked in various forms and has biological activity. Glycoprotein is one of the important biomacromolecules in organisms and widely exists in animals, plants and microorganisms, even in single-celled organisms and viruses. It has also been found in various forms and types that it is distributed in the extracellular fluid and tissue of the organism, constituting a variety of active substances in the organism. Natural glycoproteins have physiological activities such as anti-oxidation, anti-tumor, and improving immunity. Glycoproteins play an important role in various life activities such as growth and development, information transmission, immune system, and nervous system, and are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 黄日明韩玉郝慧丽杨丽红孙远明李向梅李美英
Owner SOUTH CHINA AGRI UNIV
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