Animal model construction method for female obesity research and application of animal model construction method

A technology of animal model and construction method, which is applied in animal husbandry and other fields, can solve the problems of non-specificity, difficulty in objectification, failure to form spleen deficiency syndrome, etc., and achieve good repeatability, simple modeling method, easy operation and promotion Effect

Active Publication Date: 2019-09-06
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

There are only reports of some scholars using simple high-fat diet and exhaustive swimming to create obese animal models. The method is to use high-fat diet and exhaustive swimming to damage the spleen and stomach, create internal dampness, and cause animals to suffer from poor appetite, less drinking, weight loss, This type of model still has the following obvious deficiencies: First, the repeatability and stability of the model manufacturing method is poor, there is a lack of uniform modeling standards, there are too many uncontrollable factors, and the objectivity and standardization are poor
Second, the formation of spleen deficiency is a long-term and chronic process.

Method used

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  • Animal model construction method for female obesity research and application of animal model construction method
  • Animal model construction method for female obesity research and application of animal model construction method
  • Animal model construction method for female obesity research and application of animal model construction method

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: The construction of the rat model of obesity disorder for female obesity research

[0033] (1) Experimental animals: 100 SPF grade female SD rats, 6 weeks old, weighing 180±20g, provided by Hunan Slake Jingda Experimental Animal Co., Ltd., experimental animal license number: SCXK (Xiang) 2016-0002. Animal breeding conditions: According to the circadian rhythm of natural lighting, they were raised in an SPF-level experimental animal barrier system, and the license number for the use of experimental animals was SCXK (Gan) 2017-0004. The laboratory temperature is 23±2°C, and the relative humidity is 53±3%. The animals were given free access to water and food, and the experiment was carried out after three days of adaptive feeding.

[0034] (2) Feed:

[0035] The ingredients of normal basic maintenance feed (full price nutritional feed) are: Choline Chloride 0.2%, Corn 30%, Wheat 25.5%, Molasses Yeast 2.5%, Calcium Hydrogen Phosphate 2%, Stone Powder 1%, Gro...

Embodiment 2

[0045] Embodiment 2: three-dimensional syndrome differentiation of obesity model

[0046] (1) According to the following indicators, for the obesity rat model of female obesity research established in Example 1, from the three-dimensional evaluation and statistical analysis of the obesity rat model, the syndrome types for female obesity research are distinguished. Among them, the first dimension is to look for the general condition indicators of rats to evaluate the model mice according to the four diagnostic performances of human diseases; the second dimension is the water and salt load test; the third dimension is the hematological indicators.

[0047] The first dimension is to evaluate the model mice, specifically:

[0048] Mental behavior is divided into 0-3 grades: rapid activity and bright eyes are grade 0; mild abnormalities (sluggishness, decreased spontaneous activity) are grade 1; moderate abnormalities (mental burnout) are grade 2, and obvious abnormalities (abdomin...

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Abstract

The invention relates to the technical field of biomedicine, and particularly discloses an animal model construction method for female obesity research and application of the animal model constructionmethod. The animal model construction method comprises the following steps: selecting female adult rats; feeding a high-fat high-protein high-sugar feed for the female adult rats; carrying out forcedweight-bearing swimming for 21 days from the 8th day of feeding the high-fat and high-protein feed, and carrying out forced weight-bearing swimming for 3 minutes every day; feeding the female adult rats with ice water for 21 days while carrying out forced weight-bearing swimming; and then simply feeding a sufficient amount of high-fat high-protein high-sugar feed for the female adult rats, and after the total 60 days of molding, completing molding if the weight, the body length and the Lee's index are significantly different from those of a normal control. The defects that a disease animal model for female obesity research and an obesity model syndrome type evaluation are not available at present are overcome, and a new model for normative, standard and scientific evaluation of weight loss and fat reduction traditional Chinese medicine research is provided. The method has the characteristics of simple modeling method, easiness in operation and popularization, closeness to clinic and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for constructing an animal model for female obesity research and its application. Background technique [0002] Traditional Chinese medicine believes that the internal injuries of the seven emotions cause dysfunction in the spleen, stomach, and liver in the human body, and excess fat cannot be converted into heat and excreted, and it is deposited in the body to cause obesity. Specifically, whether the metabolism of water in the body is normal or not is closely related to obesity. The spleen governs movement and transformation, and the stomach governs acceptance. Improper diet the day after tomorrow will damage the spleen and stomach, just as the "Su Wen Interpretation" says: "Eating fat will stagnate the Qi and not reach it, so the internal heat; eating sweet will slow down the Qi in the middle and retain it well, so Zhongman". Therefore, insufficient temper or...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0362
Inventor 刘志勇罗小泉李龙雪李中炼黄云杨志龙刘隆霸
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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