Animal model construction method for female obesity research and application of animal model construction method

Abstract

The invention relates to the technical field of biomedicine, and particularly discloses an animal model construction method for female obesity research and application of the animal model constructionmethod. The animal model construction method comprises the following steps: selecting female adult rats; feeding a high-fat high-protein high-sugar feed for the female adult rats; carrying out forcedweight-bearing swimming for 21 days from the 8th day of feeding the high-fat and high-protein feed, and carrying out forced weight-bearing swimming for 3 minutes every day; feeding the female adult rats with ice water for 21 days while carrying out forced weight-bearing swimming; and then simply feeding a sufficient amount of high-fat high-protein high-sugar feed for the female adult rats, and after the total 60 days of molding, completing molding if the weight, the body length and the Lee's index are significantly different from those of a normal control. The defects that a disease animal model for female obesity research and an obesity model syndrome type evaluation are not available at present are overcome, and a new model for normative, standard and scientific evaluation of weight loss and fat reduction traditional Chinese medicine research is provided. The method has the characteristics of simple modeling method, easiness in operation and popularization, closeness to clinic and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for constructing an animal model for female obesity research and its application. Background technique [0002] Traditional Chinese medicine believes that the internal injuries of the seven emotions cause dysfunction in the spleen, stomach, and liver in the human body, and excess fat cannot be converted into heat and excreted, and it is deposited in the body to cause obesity. Specifically, whether the metabolism of water in the body is normal or not is closely related to obesity. The spleen governs movement and transformation, and the stomach governs acceptance. Improper diet the day after tomorrow will damage the spleen and stomach, just as the "Su Wen Interpretation" says: "Eating fat will stagnate the Qi and not reach it, so the internal heat; eating sweet will slow down the Qi in the middle and retain it well, so Zhongman". Therefore, insufficient temper or...

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Problems solved by technology

There are only reports of some scholars using simple high-fat diet and exhaustive swimming to create obese animal models. The method is to use high-fat diet and exhaustive swimming to damage the spleen and stomach, create internal dampness, and cause animals to suffer from poor appetite, less drinking, weight loss, This type of model still has the following obvious deficiencies: First, the repeatability and stability of the model manufacturing method is poor, there is a lack of uniform modeling standards, there are too many uncontrollable factors, and the objectivity and standardization are poor

Second, the formation of spleen deficiency is a long-term and chronic process. The syndrome of spleen deficiency cannot be formed in such a short period of time. It may only be a short-term state, which is easy to recover naturally, and there is a big gap with the syndrome of traditional Chinese medicine.

Third, traditional Chinese medicine believes that the body has symptoms such as drowsiness, fatigue, and listlessness as dampness, so it is often difficult to judge dampness in experimental rats.

The above-mentioned model of spleen deficiency and damp resistance mainly uses general conditions such as body weight and blood lipid indicators as evaluation indicators, which is not specific and difficult to be objective

Fourth, there is a gap with clinical

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