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A kind of composition, preparation method and application in killing cell line

A composition, solution technology

Active Publication Date: 2022-05-24
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention provides a composition, a preparation method and its application in killing cell lines, which are used to solve the problem of in vivo stability and targeting aggregation ability of tumor-targeted drugs in the prior art. And technical defects of poor carrier degradation performance

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  • A kind of composition, preparation method and application in killing cell line
  • A kind of composition, preparation method and application in killing cell line
  • A kind of composition, preparation method and application in killing cell line

Examples

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Embodiment 1

[0040] This example is one of the examples for the preparation of BP-PEG-T7 / 5-ALA NSs.

[0041] Step 1. Preparation of BP NSs: The black scales were prepared by a liquid phase exfoliation method; wherein, the liquid phase exfoliation method was as follows: the black scales were dissolved in NMP, ultrasonically vibrated intermittently for 6 h at an intensity of 200 W, and then continued to be ultrasonicated at 0°C for 8 h. After ultrasonication, the unstripped black scale crystals were removed, the supernatant was centrifuged, and the precipitate was collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after each interval of 5s, ultrasonication is applied for 1s; the method of removing the black scale crystals that have not been peeled off is: centrifuging at a speed of 6500rpm for 20min; The centrifugation time of the supernatant was 20 min.

[0042] Step 2. Preparation of T7-PEG-NH 2 : Mal-PEG-NH 2 The solution was mixed with T7-Cys ...

Embodiment 2

[0048] This example is one of the examples for the preparation of BP-PEG-T7 / 5-ALA NSs.

[0049] Step 1. Preparation of BP NSs: black scales were prepared by liquid phase exfoliation method; wherein, the liquid phase exfoliation method was as follows: black scales were dissolved in NMP, ultrasonically vibrated intermittently at 300W intensity for 5h, and then ultrasonicated at 0°C for 12h. After ultrasonication, the unstripped black scale crystals were removed, the supernatant was centrifuged, and the precipitate was collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after every interval of 2s, ultrasonication is applied for 2s; the method of removing the black scale crystals that have not been peeled off is: centrifuging at a speed of 6000rpm for 15min; The centrifugation time of the supernatant was 15 min.

[0050] Step 2. Preparation of T7-PEG-NH 2 : Mal-PEG-NH 2 The solution was mixed with T7-Cys solution, stirred, and dried to o...

Embodiment 3

[0056] This example is one of the examples for the preparation of BP-PEG-T7 / 5-ALA NSs.

[0057] Step 1. Preparation of BP NSs: Black scales were prepared by liquid phase exfoliation method; wherein, the liquid phase exfoliation method was as follows: black scales were dissolved in NMP, ultrasonically vibrated at 260W intensity for 8h intermittently, and then continued to ultrasonically sonicate at 0°C for 6h. After ultrasonication, the unstripped black scale crystals were removed, the supernatant was centrifuged, and the precipitate was collected as BP NSs. In this embodiment, the method of ultrasonic intermittent vibration is: after every interval of 4s, ultrasonication is applied for 4s; the method of removing the black scale crystals that have not been peeled off is: centrifuging at 8000rpm for 30min; The centrifugation time of the supernatant was 30 min.

[0058] Step 2. Preparation of T7-PEG-NH 2 : Mal-PEG-NH 2 The solution was mixed with T7-Cys solution, stirred, and ...

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Abstract

The invention belongs to the field of research and development of anti-tumor targeted drugs, and in particular relates to a composition, a preparation method and its application in killing cell lines. The invention provides a composition, and the raw materials include black scale, 5-aminolevulinic acid, polyethylene glycol and transferrin receptor binding peptide. The present invention also provides a preparation method of the above-mentioned composition, and the present invention provides an application of the above-mentioned composition or a product obtained by the above-mentioned preparation method in killing SAS, HSC-2 and HSC-3 cell lines. It can be obtained through experimental determination that, compared with the positive control drug, the product prepared by the technical scheme provided by the present invention has a significant inhibitory effect on the growth and reproduction of tumor cell lines, and can further promote the apoptosis of tumor cells at the same time. A composition, a preparation method and its application in killing cell lines provided by the present invention solve the problems in the prior art that it is difficult to take into account the in vivo stability, targeting aggregation ability and carrier degradation performance of tumor targeting drugs technical flaws.

Description

technical field [0001] The invention belongs to the field of research and development of anti-tumor targeted drugs, and particularly relates to a composition, a preparation method and its application in killing cell lines. Background technique [0002] Oral squamous cell carcinoma (OSCC) is one of the malignant tumors of the head and neck, and the incidence rate is increasing year by year. At present, the treatment of the disease is mainly surgery. However, surgical treatment usually removes a large area of ​​oral tissues and organs, which seriously affects the quality of life of patients after surgery. Therefore, how to efficiently and non-invasively treat OSCC is one of the urgent clinical problems. [0003] Tumor sonodynamic therapy (SDT) is the use of ultrasound to activate sonosensitive drugs that selectively accumulate in tumor tissues. The sonosensitive drugs used for SDT treatment have problems such as their unsatisfactory in vivo stability and limited ability to ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/04A61K47/60A61K47/62A61P35/00
CPCA61K41/0033A61K47/02A61K47/60A61K47/62A61P35/00
Inventor 李楠黄盛兴南川川袁理孙海鹏陈伟璇赵跃宏
Owner SHENZHEN PEOPLES HOSPITAL