Prostate cell microcapsule for tissue engineering and preparation method thereof

A tissue engineering and prostate technology, applied in the field of medical bioengineering, can solve problems such as poor mechanical properties, achieve the effects of improving mechanical properties, inhibiting fibrosis, and prolonging survival time

Active Publication Date: 2021-09-03
SHANDONG BRANDEN MEDICAL DEVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate-polylysine-sodium alginate (APA) microcapsules are prone to tissue fibrosis due to easy adsorption of proteins and cells, and their mechanical properties are poor

Method used

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  • Prostate cell microcapsule for tissue engineering and preparation method thereof
  • Prostate cell microcapsule for tissue engineering and preparation method thereof
  • Prostate cell microcapsule for tissue engineering and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Prostate cells are placed in the sodium alginate solution that mass percent concentration is 20g / L and mix, make suspension, make every milliliter suspension contain 3 * 10 6One cell; using the Inotech IE-50R Encapsulator microcapsule instrument, using the vibrating nozzle method, by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, the cell suspension is sprayed into the state of micro-droplets with a diameter of 150-1000 μm at a concentration of 100 In mmol / L calcium chloride solution, the volume ratio of cell suspension to calcium chloride solution is 1:100. React for 8 minutes to form calcium alginate beads. After the rubber beads settle, suck out the upper calcium chloride solution, then wash 3 times with 0.9% sodium chloride injection, then put the glue beads into the dendritic stabilizer solution with a concentration of 0.5g / L (dendritic polyethylene sub- The mass ratio of amine:dendritic...

Embodiment 2

[0034] Embodiment 2: The islet cells are placed in a sodium alginate solution with a mass percentage concentration of 15g / L and mixed to make a suspension, so that each milliliter of the suspension contains 1×10 6 One cell; using the Inotech IE-50R Encapsulator microcapsule instrument, using the vibrating nozzle method, by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, the cell suspension is sprayed into the state of micro-droplets with a diameter of 150-400 μm at a concentration of 80 In mmol / L calcium chloride solution, the volume ratio of cell suspension to calcium chloride solution is 1:100. React for 5 minutes to form calcium alginate gel beads. After the rubber beads settle, suck out the upper calcium chloride solution, then wash 3 times with 0.9% sodium chloride injection, then put the glue beads into the dendritic stabilizer solution with a concentration of 0.3g / L (dendritic polyethylene sub- The mass rat...

Embodiment 3

[0035] Embodiment 3: The islet cells are placed in a sodium alginate solution with a mass percentage concentration of 25g / L and mixed to make a suspension, so that each milliliter of the suspension contains 6×10 6 One cell; using the Inotech IE-50R Encapsulator microcapsule instrument, using the vibrating nozzle method, by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, the cell suspension is sprayed into the state of micro-droplets with a diameter of 700-1000μm at a concentration of 120mmol / L of calcium lactate solution, in which the volume ratio of cell suspension to calcium chloride solution is 1:100. React for 10 minutes to form calcium alginate beads. After the rubber beads settle, suck out the upper calcium chloride solution, then wash 3 times with 0.9% sodium chloride injection, then put the glue beads into the dendritic stabilizer solution with a concentration of 0.7g / L (dendritic polyethylene sub- The ma...

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Abstract

The invention belongs to the field of medical bioengineering, and relates to a prostate cell microcapsule for tissue engineering and a preparation method thereof. The present invention adopts the structure of sodium alginate-dendritic stabilizer component-polyethylene glycol, the inner layer of which is wrapped by sodium alginate, and then dendritic polyethyleneimine and dendritic polylysine are used as stabilizing Compared with ordinary polylysine, it has a complex network structure and more cross-linking points, which improves the mechanical properties of the microcapsules. The outermost layer of the microcapsule is composed of polyethylene glycol chain segments fixed on one side of the dendritic stabilizer component, and because of its sliding properties, it can inhibit the adhesion of proteins and cells. Since polyethylene glycol is a material that tends to be "invisible" in the human body, the microcapsules of the present invention can inhibit fibrosis to a certain extent, thereby prolonging the survival time of active cells. The cell microcapsules can help prostate cancer patients overcome various physiological dysfunctions and improve the quality of life.

Description

technical field [0001] The invention belongs to the field of medical bioengineering, and relates to a prostate cell microcapsule for tissue engineering and a preparation method thereof. Background technique [0002] The prostate is an important substantive organ among the accessory glands of the male genitalia. It is an unpaired substantive gland located between the bladder and the urogenital diaphragm and surrounds the root of the urethra. Its shape and size resemble a slightly flattened chestnut. Prostatic fluid is a secretion from the prostate gland. The secretion of prostatic fluid is controlled by male hormones, and the daily secretion is about 0.5-2 ml. It is an important component of semen and one of the components of seminal plasma in semen, accounting for about 1 / 10 to 1 / 3 of the amount of ejaculated semen. The content of protein in prostatic fluid is very small, mainly containing high concentration of zinc ions, acid phosphatase, proteolytic enzyme, fibrinase, sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/18A61L27/20A61L27/50A61L27/54
CPCA61L27/18A61L27/20A61L27/3804A61L27/50A61L27/54A61L2300/622A61L2300/64C08L5/04C08L77/04C08L79/02C08L71/02
Inventor 张海军袁坤山王如蒙鲁手涛刘黎明曹文瑞张淑欣徐海荣
Owner SHANDONG BRANDEN MEDICAL DEVICE
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