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A low-yield isoamyl alcohol, high-yield β-phenylethanol yeast and its isolation and culture method and application

A technology of isoamyl alcohol and phenethyl alcohol is applied in the field of winemaking to achieve the effects of improving high-quality product rate and wine yield, excellent sugar tolerance and good ethanol production capacity

Active Publication Date: 2020-08-14
JIANGSU KINGS LUCK BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Also lack a kind of highly targeted low-yield isoamyl alcohol, high-yield beta-phenylethanol good yeast in the prior art, therefore, develop a kind of method of selection and breeding low-yield isoamyl alcohol, high-yield beta-phenylethanol good yeast, to Improving the production of Luzhou-flavored and rice-flavored liquor is of great significance to improving the high-quality rate and yield of original liquor

Method used

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  • A low-yield isoamyl alcohol, high-yield β-phenylethanol yeast and its isolation and culture method and application
  • A low-yield isoamyl alcohol, high-yield β-phenylethanol yeast and its isolation and culture method and application
  • A low-yield isoamyl alcohol, high-yield β-phenylethanol yeast and its isolation and culture method and application

Examples

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Effect test

Embodiment 1

[0049] The present embodiment provides a kind of yeast with low yield of isoamyl alcohol and high yield of β-phenylethanol, and the isolation and cultivation method comprises the following steps:

[0050] (1) Take 10 g of fermented grains fermented in Luzhou-flavor wine cellar for 5 days in a triangular flask filled with 90 mL of sterile water, and shake for 30 minutes to obtain a bacterial suspension;

[0051] (2) Dilute the bacterial suspension obtained in step (1) by the 10-fold method, apply the rolling ball method on the sterile modified YPD solid medium, and culture it upside down at 28° C. for 3 days to obtain colonies;

[0052] (3) Pick the morphologically regular bacterium colony in step (2) and streak culture on the sterile modified YPD solid medium, carry out 8 repeated operations to separate and purify, obtain a single pure-bred bacterium colony, confirm it as yeast by microscopic examination, and then 26S rDNA sequence homology analysis method was used to screen o...

Embodiment 2

[0061] The present embodiment provides a kind of yeast with low yield of isoamyl alcohol and high yield of β-phenylethanol, and the isolation and cultivation method comprises the following steps:

[0062] (1) Take 10 g of fermented grains fermented in the Luzhou-flavor wine cellar for 10 days in a triangular flask filled with 90 mL of sterile water, and shake for 30 minutes to obtain a bacterial suspension;

[0063] (2) Dilute the bacterial suspension obtained in step (1) by the 10-fold method, apply the rolling ball method on the sterile modified YPD solid medium, and culture it upside down at 30° C. for 2 days to obtain colonies;

[0064] (3) Pick the morphologically regular colony in step (2) and streak it on the sterile modified YPD solid medium, perform 10 repeated operations to separate and purify, and obtain a single pure-bred colony, which is determined to be yeast by microscopic examination, and then The same 26S rDNA sequence homology analysis method as in Example 1 ...

Embodiment 3

[0066] The present embodiment provides a kind of yeast with low yield of isoamyl alcohol and high yield of β-phenylethanol, and the isolation and cultivation method comprises the following steps:

[0067] (1) Take 10 g of fermented grains fermented in the Luzhou-flavor wine cellar for 1 day in a triangular flask filled with 90 mL of sterile water, and shake for 30 minutes to obtain a bacterial suspension;

[0068] (2) Dilute the bacterial suspension obtained in step (1) by the 10-fold method, and then apply the rolling ball method on the aseptic modified YPD solid medium and culture it upside down at 30°C for 3 days to obtain colonies;

[0069] (3) Pick the morphologically regular colony in step (2) and streak it on the aseptic modified YPD solid medium, perform 5 repeated operations to separate and purify, and obtain a single pure-bred colony, which is determined to be yeast by microscopic examination, and then The same 26S rDNA sequence homology analysis method as in Example...

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Abstract

The invention relates to a low-yield isoamyl alcohol and high-yield β-phenylethanol yeast and its isolation and cultivation method and application. The yeast with low yield of isoamyl alcohol and high yield of β-phenylethanol is named as Candida humilis (Candida humilis) YS023A strain. date, the deposit number is CGMCC No.17257, and the address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. It has the excellent characteristics of low yield of isoamyl alcohol and high yield of β-phenylethanol, and has excellent resistance to sugar, alcohol and acid, and its sugar utilization rate, fermentability and ethanol production capacity are all very good. The use of the strain in the production of Luzhou-flavored and rice-flavored liquors is of great significance for improving the high-quality product rate and liquor yield of the original liquor.

Description

technical field [0001] The invention belongs to the technical field of brewing, and specifically relates to a yeast and its separation and cultivation method and application, in particular to a low-yield isoamyl alcohol and high-yield beta-phenylethanol yeast and its separation and cultivation method and application. Background technique [0002] Baijiu is a kind of liquor that has been passed down by the Chinese for thousands of years, and it is also the most commonly drunk liquor in China. There are also many studies on its brewing technology, but now the traditional liquor brewing technology generally exists The taste is not mild enough, the flavor is not good and other disadvantages. [0003] CN105886414A discloses a high-yield organic acid Saccharomyces cerevisiae and its application in wine production. The strain is isolated from the surface of grapes in Fukang, Xinjiang, China. Separation and purification are carried out, and then after primary screening, secondary s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/02C12G3/02C12R1/72
CPCC12G3/02C12N1/02C12N1/16C12N1/165C12R2001/72
Inventor 季方吴建峰袁小转刘梦夏胡佳佳
Owner JIANGSU KINGS LUCK BREWERY
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