Non-enzymatic cellulose hydrolysis and saccharification technology
A cellulose, non-enzymatic technology, applied in the production of sugar, sugar production, glucose production, etc., can solve the problems of long ball milling time, high processing energy consumption, low concentration of sugar solution, etc., and achieve low pollution and energy consumption. Low, the effect of promoting the process of industrialization
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Embodiment 1
[0027] Preparation of lignocellulose hydrolyzate
[0028] Weigh 50g of corn stalks and 2.5g of phosphorus pentoxide, mix them in a ball mill jar, mill for 30 minutes, and then heat to 180°C for 60 minutes. Then weigh 4g of the ball-milled sample into the reactor, add 40mL of water, and hydrolyze for 40min at 215°C. After the reaction was completed, it was cooled to room temperature, and the liquid product was collected. Qualitative and quantitative analysis by HPLC results in a glucose yield of 50.1%.
Embodiment 2
[0030] Weigh 50 g of corn stalks and 5 g of phosphorus pentoxide and mix them in a ball mill jar, mill for 30 minutes, and then heat to 140° C. for 60 minutes. Then weigh 4g of the ball-milled sample into the reactor, add 40mL of water, and hydrolyze for 40min at 215°C. After the reaction was completed, it was cooled to room temperature, and the liquid product was collected. Qualitative and quantitative analysis by HPLC results in a glucose yield of 52.3%.
Embodiment 3
[0032] Weigh 50 g of corn stalks and 5 g of phosphorus pentoxide, mix them in a ball mill jar, mill for 30 minutes, and then heat to 80° C. for 60 minutes. Then weigh 4g of the ball-milled sample into the reactor, add 40mL of water, and hydrolyze for 40min at 215°C. After the reaction was completed, it was cooled to room temperature, and the liquid product was collected. Qualitative and quantitative analysis by HPLC results in a glucose yield of 45.3%.
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