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Bacillus with effect of inducing corn to be resistant to northern leaf blight of corn and application

A technology for maize large spot disease and Bacillus megaterium, which is applied in the directions of application, bacteria, and microorganism-based methods to achieve the effects of light and simple disease control methods, long control time, and high-efficiency control

Active Publication Date: 2019-09-10
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seed coating treatment is a simple and practical new method to stimulate immunity to prevent and control maize adult plant disease, which can activate the plant's defense response. At present, there is no research report on the prevention and control of maize leaf spot by the above method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of a Bacillus fermentation broth with the effect of inducing corn resistance to corn leaf spot disease

[0024] The bacillus with the effect of inducing corn resistance to corn leaf spot disease is Bacillus megaterium, the deposit number of Bacillus megaterium is CGMCC No. 17573, the depositary unit is the General Microbiology Center of China Microbial Species Collection Management Committee, and the deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, preserved on April 16, 2019, classified as Bacillus megaterium Bacillus megaterium Sneb85;

[0025] The application method of the bacillus with the effect of inducing corn resistance to corn leaf spot disease includes the following steps:

[0026] (1) Strain activation culture

[0027] Use solid medium for plate culture of Bacillus megaterium, cultivate at 28°C for 3 days to obtain a single colony;

[0028] The bacterial culture medium formula...

Embodiment 2

[0034] Field test on the effect of Bacillus megaterium Sneb85 fermentation broth on controlling corn leaf spot

[0035] Test site: Corn test field in Kangping County, Shenyang City, Liaoning Province, where corn leaf spot disease was severe in the previous year;

[0036] Corn variety: Zhengdan 958, purchased at the seed sales market.

[0037] deal with:

[0038] Treatment 1 is the seed coating treatment of Bacillus megaterium Sneb85 fermentation broth;

[0039] Treatment 2 is corn seeds without coating (CK1);

[0040] Treatment 3 is beef extract peptone liquid culture medium coating (CK2);

[0041] Coating treatment method: Coating the corn seeds with the fermentation broth of Bacillus megaterium prepared in Example 1 and adjusting the concentration; disinfect the corn seeds with 5.0% sodium hypochlorite solution for 5 minutes, and then rinse with sterile water 3 -5 times, let dry. The seed coating is carried out with a mass ratio of liquid seed of 1:40 (that is, 40 grams of corn seeds ...

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PUM

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Abstract

The invention relates to bacillus with an effect of inducing corn to be resistant to northern leaf blight of corn and an application. The bacillus with the effect of inducing corn to be resistant to northern leaf blight of corn is Bacillus megaterium, a perseveration number of the Bacillus megaterium is CGMCC No. 17573, and the preservation time is April 16, 2019; The Bacillus megaterium can induce the corn by immunization and excitation of seed treatment to produce resistance of the northern leaf blight of corn during seed germination and corn growth, activates a defensive response of the corn, stimulates immune prevention and control of the northern leaf blight of corn, and is long in time limit, does not pollute environment and soil, is a green, light and simplified new method for greenprevention and control of the northern leaf blight of corn, and has wide application and popularization prospects.

Description

Technical field [0001] The invention belongs to the technical field of microbial pesticides, and specifically relates to a bacillus with the effect of inducing corn resistance to corn leaf spot disease and its application. Background technique [0002] Northern leaf blight of corn is one of the important leaf diseases commonly occurring in corn production. It was first reported in Italy in 1876, and then spread throughout the Americas, Europe, Asia, Africa and other corn producing areas. The occurrence of corn leaf spot disease was first recorded in my country in 1899, and then spread all over the country. The disease was more severe in the cold maize producing areas in the northeast, northern north, northwest and southern mountainous areas. (Dong Jingao. Agricultural Phytopathology (Third Edition) [M]. Beijing: China Agriculture Press, 2001.) Corn leaf spot disease can cause large areas of maize leaves to die, resulting in loss of maize yield. In recent years, due to the single...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01C1/06A01C1/08C12R1/11
CPCA01C1/06A01C1/08C12R2001/11C12N1/205A01N63/22C12N1/20A01N63/20C05G3/60
Inventor 柳宁段玉玺陈立杰朱晓峰范海燕王媛媛刘晓宇闫继辰
Owner SHENYANG AGRI UNIV