Acquisition method of herbicide resistance coix lacryma resource

A herbicide-resistant and coix-resistant technology, applied in the field of plant cultivation, can solve problems such as low efficiency, and achieve the effects of simplifying the weeding process, strengthening resistance, and improving labor efficiency.

Pending Publication Date: 2019-09-10
安徽省农业科学院棉花研究所
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Problems solved by technology

Job’s tears are mainly produced in remote areas, and weeding in the field mainly depends on t

Method used

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Embodiment Construction

[0023] The technical scheme of the present invention will be further described below in conjunction with specific examples:

[0024] A method for obtaining herbicide-resistant coix resources, comprising the following steps:

[0025] 1. Aseptic embryo culture

[0026] Coix seeds from which the involucre was removed were soaked in 75% alcohol for 2 minutes, rinsed with sterile water for 3 times, and cultured on a sterile water paper bed. After germination, sterilize with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 to 8 times, cut off the germ, and inoculate it in MS medium for sterile culture at a temperature of (22±1)°C and a light intensity of 2000 lx.

[0027] 2. Callus induction

[0028] After the buds are 3cm long, slice them obliquely and transfer them to the culture medium (N 6 +2.0mg / L2,4-D+500mg / L proline+300mg / L hydrolyzed casein+1.0mg / L AgNO 3 +30g / L sucrose), pH 5.8, temperature (22±1)°C, light for 13h, 1200lx, and the original culture me...

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Abstract

The invention discloses an acquisition method of a herbicide resistance coix lacryma resource. The method comprises the following steps of (1) sterile embryo culture; (2) callus induction; (3) callusmultiplication culture; (4) transfer of anti-phosphinothricin bar gene into callus for co-culture; (5) multiplication culture of resistant callus; (6) culture and screening of the resistant callus byadopting phosphinothricin; (7) redifferentiation of anti-phosphinothricin embryonic callus; (8) seedling hardening and transplanting. Through multiple steps of scientific and accurate culturing and screening, the herbicide resistance coix lacryma resource can be efficiently obtained, the resource has high resistance to herbicides, scruples of herbicide application can be freed well, in the coix lacryma planting process, the weeding process can be simplified, and the labor efficiency is improved; the economic output is increased, and good application and popularization value is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for obtaining herbicide-resistant Coix coix resources. Background technique [0002] There are many kinds of weeds in Coix field. They have strong adaptability and strong reproductive ability. They compete with Coix for sunlight, water, fertilizer and space. Negligent management will lead to serious yield reduction. Weed removal is an important cultivation technology link in Coix production. As an integral part of the modern agricultural production system, herbicide weeding is the most reliable and economical measure in the weeding technology of coix field, and it is of great significance to save labor, improve labor productivity and protect soil structure. While herbicides eliminate weeds, they also have a harmful effect on Coix itself, which limits the application of herbicides. At the same time, the application scale of Job's tears is smaller th...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H4/00A01H5/00A01H6/46
CPCC12N15/8274C12N15/8205A01H4/001A01H4/008A01H4/005
Inventor 江本利闫晓明程福如朱加保於春路献勇王红娟胡积送
Owner 安徽省农业科学院棉花研究所
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