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Method for determining pollen viability of calligonum mongolicm Turcz by utilizing in-vitro germination liquid culture medium of calligonum mongolicm Turcz pollen

A technology of liquid culture medium and pollen viability, which is applied in the direction of biochemical equipment and methods, microbial determination/testing, measuring devices, etc., can solve the problem of not being able to well define vigor and inactivity, and not being able to distinguish pollen vigor , color difference and other issues

Pending Publication Date: 2019-09-10
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By staining fresh and burnt pollen separately, we found that the results of using these three staining agents are not very satisfactory in the plants of the genus Azalea, and the pollen viability cannot be accurately identified
I 2 -KI is darker in coloration, and cannot distinguish the vigor of the pollen very well. It can even dye the non-viable Azalea pollen into deep purple blue; after dyeing with TTC method, the color difference is not big, and it cannot be well defined Vitality and non-viability; MTT staining method has high accuracy, but the reagents are expensive, and the storage conditions of the reagents are relatively harsh. Once the reagents undergo qualitative changes, the experimental results will be seriously affected, and the experiment needs to be completed quickly

Method used

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  • Method for determining pollen viability of calligonum mongolicm Turcz by utilizing in-vitro germination liquid culture medium of calligonum mongolicm Turcz pollen
  • Method for determining pollen viability of calligonum mongolicm Turcz by utilizing in-vitro germination liquid culture medium of calligonum mongolicm Turcz pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 (taking Jinghe Shaguaizao as an example)

[0021] Preparation of medium:

[0022] a. With distilled water as solvent, pH value is 5.6, 100g / L sucrose, 32mg / L boric acid, 36.5mg / L Ca(NO 3 ) 2 , 20min after light incubation;

[0023] Pollen collection:

[0024] b. Select a strong Jinghesha Jujube plant with good growth and no diseases and insect pests, collect the whole flower branch at 8:00-9:00 in the morning during the full flowering period, bring it back, insert it in a bottle, and wait for the flower to open naturally and the pollen to disperse , carefully pick off the flowers with tweezers and collect them for later use;

[0025] In vitro pollen germination and pollen tube growth:

[0026] c. Drop the medium prepared in step a into the groove of the concave glass slide, dip the pollen obtained in step b with a brush and evenly sprinkle it on the liquid medium, place it in a petri dish with filter paper at the bottom, Put the lid on and place it in...

Embodiment 2

[0032] Embodiment 2 (taking Jinghe Shaguaizao as an example)

[0033] Preparation of medium:

[0034] a. With distilled water as solvent, pH value is 5.6, 100g / L sucrose, 32mg / L boric acid, 36.5mg / L Ca(NO 3 ) 2 , 30min after light incubation;

[0035] Pollen collection:

[0036] b. Select a strong Jinghesha Jujube plant with good growth and no diseases and insect pests, collect the whole flower branch at 8:00-9:00 in the morning during the full flowering period, bring it back, insert it in a bottle, and wait for the flower to open naturally and the pollen to disperse , carefully pick off the flowers with tweezers and collect them for later use;

[0037] In vitro pollen germination and pollen tube growth:

[0038] c. Drop the medium prepared in step a into the groove of the concave glass slide, dip the pollen obtained in step b with a brush and evenly sprinkle it on the liquid medium, place it in a petri dish with filter paper at the bottom, Cover the lid and place it in ...

Embodiment 3

[0041] Embodiment 3 (taking Jinghe Shaguazizao as an example)

[0042] Preparation of medium:

[0043] a. With distilled water as solvent, pH value is 5.6, 100g / L sucrose, 32mg / L boric acid, 36.5mg / L Ca(NO 3 ) 2 , 35min after light incubation;

[0044] Pollen collection:

[0045] b. Select a strong Jinghesha Jujube plant with good growth and no diseases and insect pests, collect the whole flower branch at 8:00-9:00 in the morning during the full flowering period, bring it back, insert it in a bottle, and wait for the flower to open naturally and the pollen to disperse , carefully pick off the flowers with tweezers and collect them for later use;

[0046] In vitro pollen germination and pollen tube growth:

[0047] c. Drop the medium prepared in step a into the groove of the concave glass slide, dip the pollen obtained in step b with a brush and evenly sprinkle it on the liquid medium, place it in a petri dish with filter paper at the bottom, Cover the lid and place it in a...

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Abstract

The invention relates to a method for determining pollen viability of calligonum mongolicm Turcz by utilizing an in-vitro pollen germination liquid culture medium of calligonum mongolicm Turcz pollen.The method is completed by the steps of preparation of the culture medium, pollen collection, in-vitro pollen germination, pollen tube growth and pollen viability determination. According to the method, the pollen of fine calligonum mongolicm Turcz is selected for in-vitro culture, and the pollen viability can be reliably and effectively determined on the basis of determination of an in-vitro germination culture system of calligonum mongolicm Turcz pollen. The pollen germination rate is used as an index of pollen viability study (it is considered as germination when the pollen tube length longer than the pollen diameter). The simple, rapid and feasible method is provided for determining the pollen viability of calligonum plants, and a basis is provided for cross breeding and reproductivebiological research of the calligonum plants.

Description

technical field [0001] The invention relates to a method for measuring the vigor of the jujube pollen by using the in vitro germination liquid medium of the jujube pollen. Background technique [0002] Polygonaceae (Polygonaceae) shrubs of the genus Calligonum L. are strong xerophytic or super xerophytic plants widely distributed in deserts and Gobi in arid regions. They are mainly distributed in the Sahara, the Mediterranean, Soviet Central Asia, Kazakhstan and Asia middle part. There are about 35 species in this genus. There are 23 species in my country, which are mainly distributed in Xinjiang, Inner Mongolia, Gansu, Qinghai and other regions. Among them, Xinjiang has the most, with 22 species. Azalea plants are one of the important construction species of sandy and partially gravel desert vegetation. They are resistant to drought, wind erosion and sand burial, fast growth, and easy to reproduce. Because of their excellent windproof and sand-fixing capabilities, they hav...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5097
Inventor 康晓珊王喜勇刘鹏索菲娅
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI