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Method for analyzing and detecting fatty acid metabolites for diagnosis of systemic sclerosis

A technology for systemic sclerosis and fatty acid metabolism, applied in the field of metabolomics, can solve problems such as not very ideal antibody markers, and achieve the effects of high sensitivity, fast analysis speed, and high resolution

Inactive Publication Date: 2019-09-10
NANJING DRUM TOWER HOSPITAL
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Problems solved by technology

Nevertheless, the currently used SSc serum autoantibody markers are not very ideal, and the positive rate of anti-Scl-70 antibody clinical detection is only 30-40%. In view of the heterogeneity and variability of the disease itself, scholars are still working on it. Serological markers for early diagnosis of SSc

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  • Method for analyzing and detecting fatty acid metabolites for diagnosis of systemic sclerosis
  • Method for analyzing and detecting fatty acid metabolites for diagnosis of systemic sclerosis
  • Method for analyzing and detecting fatty acid metabolites for diagnosis of systemic sclerosis

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with specific examples.

[0025] The present invention includes 30 patients diagnosed with systemic sclerosis. All patients met the 2013 ACR / EULAR criteria; patients with other chronic autoimmune diseases and cardiovascular diseases were excluded. 30 healthy people were selected as the control group, including 30 females, aged (31.23±4.51) years old; 30 patients with systemic sclerosis were selected as the case group, including 6 males and 24 females, aged (46.38±11.62) years old.

[0026] Clinical data collection: record the gender, age, weight and other demographic information of all research subjects; record the clinical manifestations, physical examination, vital signs, auxiliary examination and treatment plan of the patients. All subjects signed the informed consent form, and 3ml of venous blood was collected on an empty stomach in the morning, and the separated serum was stored at -80°C.

[002...

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Abstract

The invention relates to a method for analyzing and detecting fatty acid metabolites for diagnosis of systemic sclerosis. After pretreatment, samples are separated by ultra performance liquid chromatography UHPLC, wherein the chromatographic conditions are that the column temperature is 25 DEG C, the flow rate is 0.3mL / min, the sample size is 2[mu]L, the mobile phase composition A is water plus 25mM ammonium acetate plus 25mM ammonia, and the mobile phase B is acetonitrile, and the gradient elution is finally carried out. The samples are continuously analyzed in a random order, QC samples areinserted into the sample queue to monitor and evaluate the stability of the system and the reliability of the experimental data, and the samples are performed with mass spectrometry by a mass spectrometer Q-TOF after being separated by ultra performance liquid chromatography (HPLC). The experiment proves that the invention has the advantages of high analysis speed, high sensitivity and high resolution, and is suitable for the detection and analysis of fatty acid metabolites for the diagnosis of systemic sclerosis.

Description

technical field [0001] The invention relates to the field of metabolomics, in particular to a method for analyzing and detecting fatty acid metabolites for the diagnosis of systemic sclerosis. Background technique [0002] Systemic sclerosis (SSc), also known as scleroderma, is a connective tissue disease characterized by localized or diffuse skin thickening and fibrosis that can cause multisystem damage . In Asia, the incidence rate of SSc is 7.2 / 1000000-10.9 / 1000000 people, and the prevalence rate is 38 / 1000000-56.3 / 1000000 people, and the incidence of SSc is significantly higher in women than in men. According to the degree of skin involvement, SSc is clinically divided into localized SSc (lcSSc), in which skin thickening is limited to the distal end of the elbow or knee, with less involvement of internal organs; diffuse SSc (dcSSc), with extensive skin involvement, Complications such as severe visceral involvement can occur at an early stage. [0003] Fibrosis, the le...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8696G01N30/88G01N2030/8813
Inventor 王丹丹孙凌云冯学兵张华勇孙宸
Owner NANJING DRUM TOWER HOSPITAL