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Preparation method of biological polypeptide for phenylketonuria medicinal food, and use thereof

A technology for phenylketonuria and biological peptides, applied in the field of biomedicine, can solve problems such as the difficulty of separating and purifying biological peptides, and achieve the effects of achieving feasibility, ensuring nutritional needs, and reducing difficulty

Pending Publication Date: 2019-09-13
兰州天禾生物催化技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method and application of biological polypeptides for phenylketonuria medicinal food, so as to solve the problem of difficult separation and purification of biological polypeptides without alanine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the method for preparing the biological polypeptide for phenylketonuria medicinal food is as follows:

[0029] A, select whey powder with ash mass content not greater than 1.5% as the raw material for the preparation of biological polypeptides;

[0030] B, select trypsin, pepsin, chymosin combination as the biological enzyme for preparing biological polypeptide, and the combined ratio of selected trypsin, pepsin, chymosin is 1:1.5:5;

[0031] C. Adjust the pH of the material to 6.5 and the temperature to 36°C, add biological enzymes according to the ratio in step B, and after 1.5 hours of enzymolysis, keep the temperature constant, adjust the pH of the material to 4.2, and continue the enzymatic hydrolysis for 50 minutes;

[0032] D. Utilize ultrafiltration membrane technology and ion chromatography technology to separate and purify the target biological polypeptide. The ultrafiltration membrane technology is: the separation membrane uses 2500Da and 20000D...

Embodiment 2

[0036] Embodiment 2, the method for preparing the biological polypeptide for phenylketonuria medicinal food is as follows:

[0037] A, select milk powder with ash mass content not greater than 1.5% as the raw material for the preparation of biological polypeptides;

[0038] B, select trypsin, pepsin, chymosin combination as the biological enzyme for preparing biological polypeptide, and the combined ratio of selected trypsin, pepsin, chymosin is 1:1.5:5;

[0039] C. Adjust the pH of the material to 6.0 and the temperature to 36°C, add biological enzymes according to the ratio in step B, and after 1.5 hours of enzymatic hydrolysis, keep the temperature constant, adjust the pH of the material to 4.0, and continue the enzymatic hydrolysis for 50 minutes;

[0040] D. Utilize ultrafiltration membrane technology and ion chromatography technology to separate and purify the target biological polypeptide. The ultrafiltration membrane technology is: the separation membrane uses 2500Da a...

Embodiment 3

[0044] Embodiment 3, the method for preparing the biological polypeptide for phenylketonuria medicinal food is as follows:

[0045] A, select whey powder with ash mass content not greater than 1.5% as the raw material for the preparation of biological polypeptides;

[0046] B, select trypsin, pepsin, chymosin combination as the biological enzyme for preparing biological polypeptide, and the combination ratio of selected trypsin, pepsin, chymosin is 1:2:5.5;

[0047] C. Adjust the pH of the material to 6.0 and the temperature to 35°C, add biological enzymes according to the proportion in step B, and after 1.5 hours of enzymolysis, keep the temperature constant, adjust the pH of the material to 3.8, and continue the enzymatic hydrolysis for 50 minutes;

[0048]D. Utilize ultrafiltration membrane technology and ion chromatography technology to separate and purify the target biological polypeptide. The ultrafiltration membrane technology is: the separation membrane uses 2500Da and...

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Abstract

The present invention discloses a preparation method of a biological polypeptide for phenylketonuria medicinal food, and a use thereof, belongs to the field of biomedicines, and solves a problem thatseparation and purification of phenylalanine-free biological polypeptide are difficult. The preparation method comprises the following steps: one of whey powder, milk powder or fresh milk is selectedto be used as a preparation raw material; trypsin, pepsin and chymosin are selected to be combined as biological enzymes for preparing the biological polypeptide; the biological enzymes are added in the preparation raw material for enzymatic hydrolysis; and separation and purification of the target biological polypeptide are conducted, and a mass fraction of phenylalanine in the purified biological polypeptide is 0-0.15%. The amount of the phenylalanine in the biological polypeptide is controlled to be within 0.15%, avoiding a problem of difficulty in separation and purification caused by pursuing product purity and reducing industrial production costs; and the prepared biological polypeptide solves limitation requirements of patients with phenylketonuria for the phenylalanine and also ensures nutritional needs of the patients for amino acids and proteins.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method and application of a biological polypeptide used for phenylketonuria medicinal food. Background technique [0002] Phenylketonuria (PKU) is a common amino acid metabolic disease. Its phenylalanine cannot be converted into tyrosine, resulting in the accumulation of phenylalanine and ketoacids in the human body, which seriously affects the normal growth and development of patients. Manifested as mental retardation, neuropsychiatric symptoms, skin scratches, etc., patients with phenylketonuria cannot normally eat daily food, especially rice, flour, meat products and dairy products. Patients with phenylketonuria must eat special medicinal foods due to amino acid metabolism disorders, but completely restricting the intake of phenylalanine will also affect the physical development of patients. Phenylalanine is one of the 8 essential amino acids in the human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/18A23L33/18
CPCC12P21/06C07K1/36C07K1/34C07K1/18A23L33/18A23V2002/00A23V2250/55A23V2200/30
Inventor 弥超秦云骆小军朱明张调调刘慧琴
Owner 兰州天禾生物催化技术有限公司
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