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Preparation method of photo electrochemical calcitonin eobiont sensor taking flower-shaped WO3 composite material as foundation

A biosensor, tungsten trioxide technology, applied in the direction of material electrochemical variables, etc., can solve the problems of long detection period, cumbersome steps, low sensitivity, etc., and achieve the effect of improving conductivity, enhancing sensitivity and improving performance

Inactive Publication Date: 2019-09-17
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It should be noted that although there are many detection methods for procalcitonin, their quantitative determination is affected by many factors, which has the disadvantages of low sensitivity, long detection cycle, and cumbersome steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of WO 3 / NCQDs / Sb 2 S 3 The specific steps are:

[0033] (1) Add 9 mmol of Na 2 WO 4 ∙2H 2 O was dissolved in 16.36 mL of ultrapure water under continuous stirring, and then 4.9 mol / L of HCl solution was added dropwise to the above solution to adjust its pH value to about 1.5, and then 18 mmol of NaCl and 0.83 mmol of h 2 C 2 o 4 Add to the above mixed solution, stir continuously for 30 min, then add 41 mL of ultrapure water, finally, transfer the obtained solution to a 100 mL polytetrafluoroethylene-lined autoclave, heat at 150 °C for 1 h, and cool to At room temperature, the resulting mixture was centrifuged three times with ultrapure water and ethanol, and dried in vacuum to obtain WO 3 Yellow powder, dissolved in ultrapure water to obtain WO 3 suspension;

[0034] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol, and ultrapure water for 30 min, and dry it in an oven at 140 °C for 2...

Embodiment 2

[0036] Example 2 prepares WO 3 / NCQDs / Sb 2 S 3 The specific steps are:

[0037] (1) Add 10 mmol of Na 2 WO 4 ∙2H 2 O was dissolved in 18.2 mL of ultrapure water under continuous stirring, and then 5.45 mol / L of HCl solution was added dropwise to the above solution to adjust its pH value to about 1.5, and then 20 mmol of NaCl and 0.9 mmol of h 2 C 2 o 4 Add to the above mixed solution, stir continuously for 30 min, then add 45.45 mL of ultrapure water, finally, transfer the obtained solution to a 100 mL polytetrafluoroethylene-lined autoclave, heat at 150 °C for 1 h, and cool to At room temperature, the resulting mixture was centrifuged three times with ultrapure water and ethanol, and dried in vacuum to obtain WO 3 Yellow powder, dissolved in ultrapure water to obtain WO 3 suspension;

[0038] (2) The ITO electrode was cut to a size of 2.5 cm × 0.8 cm, ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, dried in an oven at 140 °C for 2 h, an...

Embodiment 3

[0040] Example 3 prepares WO 3 / NCQDs / Sb 2 S 3 The specific steps are:

[0041] (1) Add 11 mmol of Na 2 WO 4 ∙2H 2 O was dissolved in 20 mL of ultrapure water under continuous stirring, and then 6 mol / L HCl solution was added dropwise to the above solution to adjust its pH value to about 1.5, and then 22 mmol of NaCl and 1 mmol of h 2 C 2 o 4 Add to the above mixed solution, stir continuously for 30 min, then add 50 mL of ultrapure water, finally, transfer the obtained solution into a 100 mL polytetrafluoroethylene-lined autoclave, heat at 150 °C for 1 h, and cool to At room temperature, the resulting mixture was centrifuged three times with ultrapure water and ethanol, and dried in vacuum to obtain WO 3 Yellow powder, dissolved in ultrapure water to obtain WO 3 suspension;

[0042] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry it in an oven at 140 °C for 2 h. Dissolve 10...

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PUM

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Abstract

The invention relates to a preparation method of a photo electrochemical calcitonin eobiont sensor taking a flower-shaped WO3 composite material as a foundation, and belongs to the field of a photo electrochemical sensor. With the adoption of a method of hydrothermal synthesis, unique flower-shaped WO3 is synthesized, relatively large specific area is endowed by a porous structure, nitrogen-doping carbon quantum dots (NCQDs) are used for sensitizing, and the photo electrochemical activity of the WO3 is improved; Sb2S3 is grown in an in-situ way by reaction of Sb<3+> and S<2-> and is used as a signal amplification material, and the stable flower-shaped WO3 / NCQDs / Sb2S3 composite material of which a photoelectric signal is remarkably improved is fabricated; and by a layer-by-layer self-assembly method and specific binding between an antigen and an antibody, the photo electrochemical calcitonin eobiont sensor simple to operate is built, the ultra-sensitive detection of calcitonin is achieved, and the method is of important significance to analysis and detection of the calcitonin.

Description

technical field [0001] The invention relates to a preparation method of a photoelectrochemical procalcitonin biosensor based on a flower-shaped tungsten trioxide composite material. The electrochemical workstation is used for testing with a three-electrode system, and an LED lamp is used as a photoelectrochemical luminescence signal source. Tungsten trioxide composite material WO 3 / NCQDs / Sb 2 S 3 As the base material, a WO-based 3 / NCQDs / Sb 2 S 3 The invention relates to a composite material-based photoelectrochemical procalcitonin biosensor, which belongs to the field of photoelectrochemical sensors. Background technique [0002] As a protein highly related to the immune system, procalcitonin PCT plays an important role in the human body. Its concentration increases when bacteria, fungi, and parasites invade the body, while the body's autoimmunity, allergies, and viral infections do not cause this to happen. For the above reasons, procalcitonin can be a marker for d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 范大伟刘昕鲍春竹张勇王欢魏琴王雪莹吴丹马洪敏胡丽华孙旭
Owner UNIV OF JINAN
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