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Application of carbon-based nanomaterials in the preparation of drugs for alleviating or treating hd

A carbon-based nanomaterial and inhibitor technology, which can be used in nanomedicine, drug combination, nanotechnology, etc., and can solve problems such as difficulties

Active Publication Date: 2022-04-15
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although people have placed great hopes on the application of nanomaterial drugs as "smart" drugs in HD treatment, the etiology of HD has not yet been fully elucidated, and HD treatment drugs are difficult to pass through the blood-brain barrier. Difficulty, looking at various researches, finding HD diagnosis and effective intervention methods requires courage and innovative thinking, and requires the tireless pursuit of researchers

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  • Application of carbon-based nanomaterials in the preparation of drugs for alleviating or treating hd
  • Application of carbon-based nanomaterials in the preparation of drugs for alleviating or treating hd
  • Application of carbon-based nanomaterials in the preparation of drugs for alleviating or treating hd

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preparation example Construction

[0036] The preparation method of the carbon-based nanomaterial of the present invention is as follows: react vitamin solution or retinoid solution at 170°C to 190°C for 1.5h to 2.5h; then naturally cool to room temperature and then filter; then dialyze the filtrate and freeze-dry to obtain Carbon-based nanomaterials, called CDs.

[0037] The descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible from the teachings of the invention. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling others skilled in the art to make and use various exemplary embodiments of the invention, as well as various Choose and change. It is intended that the scope of the...

Embodiment 1

[0038] Example 1 Preparation of carbon-based nanomaterials (CDs)

[0039] Weigh 1.00 g L-Vitamin C (L-Vc) and dissolve it in 10 mL H 2 O, sonicate for 20 min to fully dissolve; transfer the dissolved Vc solution to a hydrothermal kettle, react at 180 °C for 2 h, cool to room temperature naturally after the reaction, and then filter the reaction solution with a sandstar funnel to remove insoluble particles. Then use a 500-1000 Da dialysis bag to purify in water, and finally obtain a brown-red CDs solution; freeze the brown-red CDs solution in a -80 °C refrigerator for 2 h, and then use an Alpha1-4LSCplusRC6 freeze dryer at -80 °C under vacuum Freeze-dried at 10 Pa for 48 h to obtain carbon-based nanomaterials CDs. Dissolve the obtained carbon-based nanomaterial CDs in pure water to obtain an aqueous solution of carbon-based nanomaterial CDs, which is used in Examples 2 to 4.

[0040] Ultraviolet Spectrum: Dilute the CDs aqueous solution to a certain concentration, transfer it...

Embodiment 2

[0046] Example 2 CDs effectively inhibit mHtt aggregation

[0047] Transmission Electron Microscopy Cell samples: Neuro-2a (N2a) cells with C 2 N materials were co-incubated in serum-free DMEM medium for 24 hours, fixed the cells with glutaraldehyde for 10 minutes, scraped off the cells and dripped them on the copper grid, and carried out negative staining with 2% phosphotungstic acid. Photographed under an electron microscope. Protein sample: drop mHtt monomer (100 μM) on the copper grid and let it stand for 2 minutes, absorb excess sample with filter paper, wash biological samples twice with ultrapure water, negatively stain the sample with 2% phosphotungstic acid for 2 min, absorb with filter paper Remove excess phosphotungstic acid and dry overnight.

[0048] Laser confocal detection of CDs entering the nucleus CDs were incubated with N2a cells for 12 hours, the medium was aspirated, and the nuclear dye Red Dot1 was added to incubate for 10 minutes, and then imaged and o...

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Abstract

The invention discloses the application of carbon-based nanomaterials in the preparation of drugs for relieving or treating HD, and solves the problem of high synthesis cost of existing carbon nanomaterials such as fullerenes. The carbon-based nanomaterials have good water solubility, degradability and It can effectively inhibit the aggregation of mutant huntingtin protein mHtt and other significant advantages; through cell experiments and animal experiments, it was found that carbon-based nanomaterials can alleviate the toxicity of mHtt aggregation to neurons and improve the learning and memory ability of HD model mice.

Description

technical field [0001] The invention belongs to nano-medicine technology, and in particular relates to the application of a carbon-based nano-material in the preparation of drugs for alleviating or treating HD. Background technique [0002] Huntington's disease (HD) is a late-onset autosomal dominant neurodegenerative disease, the main pathological features are extensive neuronal dysfunction and selective striatal neuron degeneration, manifested as Severe damage to the small ganglion cells with gliosis, prominent pathology and atrophy of the cortex. [0003] There is a CGA trinucleotide repeat sequence in the first exon of the HD gene, and its encoded product is a polyglutamine fragment (Poly-Q) at the N-terminus of huntingtin (Htt). In normal people, HD gene The repeat number of CAG was less than 35, and the normal Htt (WT) was diffusely distributed in the cells. The mutated HD gene encodes mutant huntingtin (mutanthuntingtin, mHtt) with a poly-Q structure and misfolds. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/44A61K31/375A61P25/14
CPCA61K33/44A61K31/375A61P25/14B82Y5/00B82Y40/00
Inventor 杨再兴尹秀华李灏杨莹张梦玲李友云康振辉周如鸿
Owner SUZHOU UNIV
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