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A method for step-by-step extraction of proteins and polysaccharides in selenium-enriched Viola leaf broken rice chestnuts

A technology of crushing cordylae leaves and selenium-enriched cordieri leaves, which is applied in the field of food processing to achieve the effects of low energy consumption in the operating environment, high protein content, and shortened reaction time

Active Publication Date: 2020-08-14
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction of plant active ingredients by high-voltage pulsed electric field has not been popularized and utilized. So far, there has been no report of extracting polysaccharides by ultrasonically extracting protein from raw materials and then using high-voltage pulsed electric field.

Method used

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  • A method for step-by-step extraction of proteins and polysaccharides in selenium-enriched Viola leaf broken rice chestnuts
  • A method for step-by-step extraction of proteins and polysaccharides in selenium-enriched Viola leaf broken rice chestnuts
  • A method for step-by-step extraction of proteins and polysaccharides in selenium-enriched Viola leaf broken rice chestnuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Pulverize the selenium-enriched Corydalis sativa and sieve it through a 100-mesh sieve, take 1g of selenium-enriched sliver of Corydalis sativa, add 30g of water to mix, adjust the pH of the solution to 9-10, and perform ultrasonic extraction at 40°C, The ultrasonic energy density is 8W / mL, the extraction time is 30min, and then centrifuged at 4000r / min for 15min to obtain the first supernatant and the first precipitate, and the first supernatant is the selenoprotein extract.

[0046] (2) Add 30g of water to dissolve the first precipitate, and perform high-pressure pulse treatment. The treatment conditions are: the speed of the peristaltic pump is 38r / min, the pulse electric field strength is 6.67kV / cm, the number of pulse treatments is 27 times, and then centrifuged at 4000r / min for 15min. Obtain the second supernatant, deproteinize the second supernatant with Savage reagent, then add 4 times the volume of the second extract in absolute ethanol to precipitate overni...

Embodiment 2

[0048] The difference with Example 1 is:

[0049] In step (1), the time of ultrasonic extraction is 20min;

[0050] In step (2), the pulse electric field strength is 10kV / cm, and the number of pulse treatments is 18 times.

Embodiment 3

[0052] The difference with Example 1 is:

[0053] In step (1), the temperature of ultrasonic extraction is 50°C, and the extraction time is 40min;

[0054] In step (2), the number of pulse processing is 9 times.

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Abstract

The invention discloses a step-by-step method for extracting proteins and polysaccharides in selenium-enriched cinnamon-leaf broken catkins, comprising the following steps: pretreatment of cinnabar-leaf broken catkins, ultrasonic-assisted extraction, centrifugation, high-voltage pulse electric field secondary extraction, removal of Protein, alcohol precipitation, etc. The protein and polysaccharide extracts prepared by the two-step method have a high yield and are rich in organic selenium, and can be comprehensively developed and utilized as selenium-enriched food raw materials or nutritional enhancers. At the same time, this method extracts the protein in selenium-enriched Viola leaf broken rice catkins by ultrasonic technology, and then uses high-voltage pulse electric field to prepare selenium polysaccharide after adding water to the filter residue. The extraction process has low energy consumption and avoids the use of organic solvents. It can be used in most laboratories. Repeated realization has the potential of industrialized production.

Description

technical field [0001] The invention belongs to the field of food processing, and more specifically relates to a method for step-by-step extraction of proteins and polysaccharides in selenium-enriched corduroy leaf broken rice catkins. Background technique [0002] Selenium is an essential trace element in humans and animals. It participates in the synthesis of coenzymes and has the functions of anti-oxidation, anti-aging, anti-cancer, and antagonizing the toxicity of heavy metals. Selenium deficiency can cause more than 40 diseases such as Keshan disease and Kaschin-Beck disease. At present, there are more than 40 countries lacking selenium in the world. Studies have shown that organic selenium mainly exists in selenoproteins and selenium polysaccharides. Extracting and enriching selenium-containing active ingredients has very important theoretical and practical significance for the development of selenium-enriched functional foods. [0003] Violet-leaved Michelina is a cr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07K1/14
CPCC07K1/145C08B37/0003
Inventor 祝振洲李书艺吴麦玲程水源丛新于添
Owner WUHAN POLYTECHNIC UNIVERSITY
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