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A kind of preparation method of high tenacity silk fibroin gel

A silk fibroin, high-toughness technology, applied in the field of high-toughness gel and its preparation, can solve problems such as detrimental gel biocompatibility, and achieve the effects of less inflammatory response, increased work efficiency, and improved biosafety

Active Publication Date: 2022-02-08
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method does not need to add cross-linking agent to prepare silk fibroin gel, but adjusts the pH value and introduces a large amount of H + ions, still impairs the biocompatibility of the gel

Method used

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  • A kind of preparation method of high tenacity silk fibroin gel
  • A kind of preparation method of high tenacity silk fibroin gel
  • A kind of preparation method of high tenacity silk fibroin gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare silk fibroin solution. Specifically: put 10g silk in 0.02M Na 2 CO 3 Boil in the solution at 100°C for 30 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat the above operation 3 times, and then dry the silk at 60°C. 27 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 9.3 mol / L, and dissolved at 60° C. for 6 hours. Then use a dialysis bag with a molecular weight cut-off of 3500 to soak in deionized water for dialysis for 3 days, during which the water is changed every two hours to remove LiBr in the solution, thereby obtaining a pure silk fibroin solution.

[0029] After the silk fibroin solution was concentrated to 30% at 60°C, it was diluted to 1% with deionized water, placed in an oven for 24 hours, and then ultrasonicated for 30 seconds with an ultrasonic cell disruptor to obtain a silk fibroin nanofiber solution.

[0030] Adjust the concentr...

Embodiment 2

[0033] The pure silk fibroin solution and the silk fibroin nanofiber solution were prepared by the same method as in Example 1.

[0034] Adjust the concentration of ordinary silk fibroin solution to 9%, and then mix it with 1% crystalline silk fibroin solution at room temperature at a volume ratio of 1:1, and then suck it into a cylindrical mold, and obtain silk after standing still. Vegan Protein Gel Sticks. Take out the gel stick and bend both ends, the gel stick can be bent without breaking.

[0035] See figure 2 , figure 2 It is the anti-bending condition image of silk fibroin high tenacity gel. From figure 2 It can be seen that the gel has strong toughness.

Embodiment 3

[0037] The pure silk fibroin solution and the crystalline silk fibroin solution were prepared by the same method as in Example 1.

[0038] Dilute the ordinary silk fibroin solution to 3%, and then mix it with 1% crystalline silk fibroin solution at a volume ratio of 1:1 at 60°C to obtain a silk fibroin high-toughness hydrogel.

[0039] The 2% ordinary silk fibroin solution is ultrasonically treated, and the ordinary silk fibroin hydrogel is obtained after standing still.

[0040] See image 3 , image 3 It is a comparison chart of silk fibroin high-toughness gel with the same protein concentration described in Example 3 and ordinary silk fibroin gel. The high-toughness gel has higher transparency.

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Abstract

The invention discloses a preparation method of high tenacity silk fibroin gel. Ordinary silk fibroin solution undergoes crystallization-inducing treatment to obtain crystalline silk fibroin solution, then mixes it with ordinary silk fibroin solution, and finally obtains high-tenacity silk fibroin gel under the action of self-assembly. This technology can control the gelation time, toughness and other properties of hydrogel by changing the concentration of silk fibroin solution, the size and content of crystalline silk fibroin, self-assembly temperature and other parameters. The preparation process of the present invention is mild, no toxic organic reagents such as cross-linking agents are needed, the preparation conditions are green and controllable, and there is no waste residue, which provides a new method for the preparation of functional hydrogels and can be used in biomedicine and tissue engineering and other fields.

Description

technical field [0001] The present invention relates to a high-toughness gel and a preparation method thereof, in particular to a preparation of a flexible and high-toughness silk fibroin hydrogel which uses silk fibroin as a raw material and does not require organic solvents or other additives technology. Background technique [0002] Silk fibroin is a kind of natural protein material. The regenerated silk fibroin material has good biocompatibility, biodegradation speed and adjustable physical and chemical properties, which makes this material widely used by researchers as a scaffold material for tissue repair. focus on. [0003] Silk fibroin hydrogel has a structure similar to extracellular matrix and is a tissue repair material with good biocompatibility. However, hydrogels usually have weak mechanical properties, poor flexural strength, and are brittle and easy to break. The main reason is that its internal polymer chain density is low, there is less entanglement betw...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00
CPCC08J3/075C08J2389/00C08J2489/00
Inventor 姚丹语史廷春
Owner HANGZHOU DIANZI UNIV
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