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A drug-resistant and stress-resistant nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application

A technology of bradyrhizobia and microbial strains, applied in the field of microorganisms, can solve the problems of restricting the application of rhizobia, lack of adaptability, and lack of application effect of rhizobia strains

Active Publication Date: 2021-08-06
安徽微壤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems are: the lack of adaptable and efficient nitrogen-fixing rhizobia strains, the lack of research on the application effect of rhizobia strains in different ecological environments, the lack of rhizobia formulations and application technologies suitable for the current planting methods, etc. These problems are serious Restricts the application of rhizobia

Method used

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  • A drug-resistant and stress-resistant nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application
  • A drug-resistant and stress-resistant nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application
  • A drug-resistant and stress-resistant nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Isolation and functional identification of Bradyrhizobium HHPB1

[0067] The present invention uses the peanut rhizobium isolated and identified in Tangshan, Hebei as materials, measures the nitrogen fixation ability and adaptability (drug resistance, drought resistance, salt-alkali resistance, etc.) Efficient nitrogen-fixing peanut rhizobia.

[0068] 1. Materials and methods

[0069] 1. Experimental materials

[0070] (1) strain

[0071] The bradyrhizobium was collected, classified and identified from Tangshan, Hebei in the early stage of our laboratory.

[0072] (2) Media and reagents used

[0073] ①YMA medium

[0074] Mannitol 10.0g, K 2 HPO 4 0.25g, KH 2 PO 4 0.25g, MgSO 4 0.1g, NaCl 0.1g, yeast extract 3.0g, agar 15g (plus solid medium), deionized water 1L, pH 6.8-7.0, sterilized at 121°C for 20min.

[0075] ②TY medium

[0076] Tryptone 5.0g, yeast extract 3.0g, CaCl 2 0.6g, 15g of agar (plus solid medium) 1L of deionized water, pH 6.8-7...

Embodiment 2

[0129] Embodiment 2, field application research of bradyrhizobium HHPB1

[0130] 1. Field application method of Bradyrhizobium HHPB1

[0131] 1. Strain activation and expansion culture: About 15 days before sowing, the Bradyrhizobium HHPB1 strain stored in the refrigerator at -80°C was activated on the YMA solid medium by three-section line method, and cultivated in a constant temperature incubator at 28°C for 7 days. Isolated single colonies. Use a sterile bamboo stick to pick a single colony into TY liquid medium for expansion culture, place it at 28°C, and shake it at 180 rpm until the bacterial liquid OD 600 =1.2, the seed solution was obtained. Then seed liquid is inoculated in the new TY liquid culture medium according to the ratio of 5% (volume fraction), cultivates 3-5 days at 28 ℃ of 180rpm incubators, obtains bacterial liquid (with the non-inoculated culture medium as blank control, bacterium Liquid concentration is about 1 x 10 10-11 cfu / ml).

[0132] 2. Before...

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Abstract

The invention discloses a drug-resistant and stress-resistant nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application. The present invention provides Bradyrhizobium sp. HHPB1, the preservation number of which is CGMCC No. 17501 in China General Microorganism Culture Collection and Management Center. The bradyrhizobium HHPB1 of the present invention has wide adaptability, such as tolerance to drought, pesticide and acid, etc., and is a biosafe strain. In the Huanghuai area, it can significantly increase the ground fresh weight, the number of nodules, and the number of needles of peanuts at different development stages, and improve the fruit filling rate to a certain extent, and the peanut yield can increase by up to 27.6%. The bradyrhizobium HHPB1 described in the present invention is a stable and highly efficient microbial fertilizer strain resource for weight loss and efficiency enhancement.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a drug-resistant, stress-resistant and nitrogen-fixing bradyrhizobium strain suitable for the Huanghuai region and its application. Background technique [0002] Nitrogen fertilizer is one of the essential fertilizers in crop production, especially legumes have a greater demand for nitrogen fertilizer. Peanut is an important grain and oil crop in my country's agricultural production, and the increase in its output is material input-dependent. my country's peanut production has always relied on a large amount of chemical fertilizer input, and excessive use of chemical fertilizers will not only cause a large amount of waste of resources but also cause a series of environmental problems, such as: damage to the soil and soil microbial community structure, groundwater pollution and greenhouse gas emissions. In order to develop green agriculture, reducing the use of chemical fertilizers ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08A01C1/06C12R1/01
CPCA01C1/06A01N63/00C05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 隋新华尚佼颖吴月霍斌李永华陈腊马钧
Owner 安徽微壤生物科技有限公司