A method for site-specific editing of ccr5 gene

A gene editing technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as uncontrollable, risky, fatal safety, etc.
CN110257424BActive Publication Date: 2022-03-04广东赤萌医疗科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广东赤萌医疗科技有限公司
Publication Date
2022-03-04

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Abstract

The invention relates to the technical field of immunity to infectious diseases, in particular to a method for site-directed editing of the CCR5 gene. The present invention constructs a gRNA vector based on the CRISPR / Cas9 system based on the hTRIM5 / CypA gene and the human CCR5 gene; then co-transfects the gRNA vector based on the CRISPR / Cas9 system and the homologous recombination donor system into a recipient cell line, Thus, a cell line in which the CCR5 gene is site-specifically inserted into the hTRIM5 / CypA gene is obtained. Since the CCR5 site is an open site, the inserted exogenous hTRIM5 / CypA will continue to express stably without expressing the CCR5 gene, so that the cells can not only resist the infection of R5 phagocytic HIV‑1 virus, but also resist Infection by X4 phagocytic HIV virus.
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Description

technical field

[0001] The invention relates to the technical field of infectious disease immunity, in particular to a method for site-directed editing of CCR5 gene. Background technique

[0002] HIV-1 is an RNA virus that can infect humans and cause human immunodeficiency. After the HIV-1 virus invades the human body, it binds to the CD4 receptor on the cell surface, enters the CD4 T lymphocyte with the participation of the co-receptor CCR5 or CXCR4, and proliferates and replicates in the cell, causing a large loss of CD4 T lymphocytes. Released into the blood to form HIVemia. After the human body is infected with HIV, it will lead to immunodeficiency and cause a series of opportunistic infections and tumors, with a high mortality rate. Clinically, combined drugs are mainly used to treat AIDS, that is, highly active anti-retroviral therapy (HAART), which is commonly known as "cocktail therapy". Cocktail therapy usually consists of two reverse transcriptase inhibitors and...

Claims

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