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Method for extracting geosmin

A technology of geosmin and agricultural products, applied in chemical instruments and methods, preparation of test samples, preparation of organic compounds, etc., to achieve the effects of no secondary pollution, low material cost, and shortened production time

Inactive Publication Date: 2019-09-24
XIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no relevant reports on the extraction of geosmin from agricultural products such as beetroot

Method used

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  • Method for extracting geosmin
  • Method for extracting geosmin
  • Method for extracting geosmin

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Select fresh beetroot and wash it in clear water to remove surface stains, after chopping, take 100g and add it to a 500ml beaker for later use;

[0055] Add 200ml of 1.5% sodium chloride solution to the beaker, transfer the mixture to a blender, stir at 200rpm for 2min, adjust the speed to 1000rpm for beating for 5min, and centrifuge at 3000rpm for 10min to obtain the supernatant.

[0056] Take 100ml of the supernatant and add an equal volume of diethyl ether, put it on a vortex instrument at 500rpm for 30min, and let it stand for 10min to separate the aqueous phase and the organic phase;

[0057] Take 50ml of the organic phase and add 1g of anhydrous sodium sulfate to a 250ml beaker, seal it with a parafilm and let it stand for 8 hours for dehydration. Use a syringe to filter the mixed solution through a 0.22μm organic phase filter membrane, and concentrate the filtrate to 500μL by rotary evaporation under reduced pressure at 45°C , to obtain the geosmin extract and s...

Embodiment 2

[0060] Select fresh beetroot and wash it in clear water to remove surface stains, after chopping, take 300g and add it to a 1000ml beaker for later use;

[0061] Add 600ml of 1.5% sodium chloride solution to the beaker, transfer the mixture to a blender, stir at 300rpm for 2min, adjust the speed to 800rpm for beating for 5min, and centrifuge at 3000rpm for 10min to obtain the supernatant.

[0062] Take 300ml of the supernatant and add an equal volume of diethyl ether, place it on a vortex instrument at 700rpm for 30min, and let it stand for 10min to separate the aqueous phase and the organic phase;

[0063] Take 200ml of the organic phase and add 1g of anhydrous sodium sulfate to a 500ml beaker, seal it with a parafilm and let it stand for 8 hours to dehydrate, filter the mixed solution through a 0.22μm organic phase filter membrane with a syringe, and concentrate the filtrate to 1000mL by rotary evaporation under reduced pressure at 45°C , to obtain the geosmin extract and st...

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Abstract

The invention relates to a method for extracting geosmin from beetroot. The method comprises the steps that S1, raw materials are prepared firstly, wherein the fresh beetroot is selected and cleaned in water to remove surface stains; S2, the beetroot in S1 is placed in a sodium chloride solution which is 1-2 times the volume of the beetroot and crushed by a stirrer, and a supernatant is kept after centrifugation; S3, the supernatant is taken, an equal volume of diethyl ether is added, and a mixture is placed on a vortex instrument for shaking and mixing extraction; S4, an organic phase in S3 is taken, dehydrated with anhydrous sodium sulfate and then concentrated through rotary evaporation under reduced pressure to prepare a sample, and the sample is preserved at 4 DEG C. The method for extracting the geosmin is simple in step, safe to operate, low in production raw material price and high in content and can greatly reduce the production cost.

Description

technical field [0001] The invention relates to the technical field of extraction of secondary metabolites, in particular to a method for extracting geosmin from agricultural products such as beet roots. Background technique [0002] Geosmin, a representative odorant of earthy mustiness, was first isolated and identified in actinomycetes (Gerber and Lechevalier, 1965), and subsequently in many species, such as cyanobacteria (Agger et al., 2008; Giglio et al. ,2008; Ludwig et al.,2007) and myxobacteria (Schulz et al.,2004), even found in some higher plants (Marsili et al.,1994). In addition, due to its low odor threshold (4-10ng / L) and it is difficult to remove it by conventional means (Ando et al., 1992), it has become an important source of water odor. Therefore, the detection and removal of geosmin in the environment has always been a hot spot in the study of odorous substances, and the demand for geosmin standard products has also risen for a time. At present, the synth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C29/76C07C29/86C07C29/78C07C35/36G01N1/28G01N30/02G01N30/06
CPCC07C29/76C07C29/78C07C29/86C07C2602/28G01N1/28G01N30/02G01N30/06G01N2030/062C07C35/36
Inventor 周维成杨德俊卞杰松
Owner XIANGNAN UNIV
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