Method for detecting antifungal property of coated antibacterial ceramic by ATP bio-fluorescence lgCB-lgIB standard curve method

Abstract

The invention relates to a method for detecting antifungal property of coated antibacterial ceramic, and the detection steps include: sample preparation and pretreatment; equipment selection, and reagent and medium preparation; species preservation, activation and preparation of bacterial suspension; counting of bacterial suspension live bacteria content CB blood cell plate after methylene blue staining and inoculated bacteria liquid CB calibration; lgCB-relative fluorescence intensity logarithm value lgIB standard curve establishment; sample inoculation, culture and elution recovery; IB determination of recovered liquid and live bacteria content CB and TB reckoning; antibacterial rate R or antibacterial activity value A calculation; and results evaluation. The method is characterized in that the accurate quantitative test is carried out on the antibacterial property of the glass characterized by R or A by using an ATP fluorometer. The method prescribes that of a control sample and anantibacterial sample after contact for 0 h and culture for 24 h are subjected to elution and recovery, the recovery liquid IB is determined and R or A is characterized and calculated by lgIB; and theresult evaluate basis is provided. The method for detecting antifungal property of coated antibacterial ceramic developed by the invention supports the improvement of product quality and market orderby an advanced detection technology.

Description

technical field [0001] The present invention relates to the antifungal performance test method of antibacterial ceramics, specifically a kind of ATP bioluminescent IgC that uses ATP fluorescence photometer to carry out accurate quantitative detection to the antifungal performance of antibacterial ceramics characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of ceramic antibacterial function detection. Background technique [0002] China has a long history of ceramic production. The annual output of ceramics accounts for about 90% of the world's total output, and the annual export volume exceeds 4 billion pieces; among them, daily-use ceramics, architectural ceramics, and sanitary ceramics account for 65%, 60% and 40% of the world's total output respectively. , but the total output value of ceramics is only 10% of the world's total output value, and the homogenization competition caused by l...

AI Technical Summary

Problems solved by technology

In view of the current development trend of quantification, rapidity and simplification in the research field of foreign antibacterial material performance testing technology, the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-antibacterial finishing textiles) Determination of the antibacterial performance of textiles) and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence determination of anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0, and it is not suitable for key technologies such as the recovery method of viable bacteria and the effective conditions of the test. Applicable to ceramics, glass and other inorganic non-metallic materials with non-absorbent hard surface and control fungal growth value < 0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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