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Preparation method of blood-pressure-lowering rice material, adopted gene and adopted protein

A technology for lowering blood pressure and blood pressure, which is applied in the field of genes and proteins, and can solve the problems of high cost of ACEIP, inability to realize industrialization, and low content of ACEIP

Active Publication Date: 2019-10-11
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although food proteins widely contain ACEIPs that can inhibit the increase in blood pressure, the formation of these ACEIPs into active peptides that have the effect of lowering blood pressure requires a complex proteolytic hydrolysis process. The protease hydrolyzes to form active peptides with physiological functions to exert the function of lowering blood pressure
At the same time, the content of ACEIP in natural protein is very low, and the production of ACEIP by proteolysis and hydrolysis of natural protein is very costly and cannot be industrialized

Method used

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  • Preparation method of blood-pressure-lowering rice material, adopted gene and adopted protein
  • Preparation method of blood-pressure-lowering rice material, adopted gene and adopted protein
  • Preparation method of blood-pressure-lowering rice material, adopted gene and adopted protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Example 1. Preparation of blood pressure-lowering-related proteins and genes encoding them

[0112] This example provides a protein with hypotensive function linked by ten kinds of angiotensin-converting enzyme inhibitory peptides (ACEIP). The protein is named as hypotensive-associated protein (SPAA). 3-8, 11-16, 19-30, 31-33, 34-40, 43-47, 50-61, 64- At positions 66, 68-73 and 76-85, the two ACEIPs that cannot be digested by pepsin are connected by glutamine and arginine, so that the two polypeptides that cannot be digested by pepsin can be Digested with trypsin, the amino acid sequence of SPAA is sequence 1 in the sequence listing.

[0113] The nucleotide sequence encoding SPAA was artificially designed according to the rice codon preference, and the DNA fragment was recorded as the SPAA gene. Synthesis of the SPAA gene.

Embodiment 2

[0114] Example 2. Preparation of new transgenic rice material for lowering blood pressure and detection of its blood pressure lowering function

[0115] 1. Construction of binary plant expression vectors

[0116] The DNA molecule shown in sequence 2 in the sequence list is artificially synthesized, the 1-105th position of sequence 2 is the GluA2 signal peptide sequence, the 106-360th position and the 361-615th position are all SPAA gene sequences, the sequence 2 shown in The DNA fragment was designated GluA2-2SPAA gene.

[0117] The pGPTV-pGluC-tGluB5 expression vector (Wen Jing Li Ling Ling Dai Zhi JianChai Zhi Jie Yin Le Qing Qu. Evaluation of seed storage protein gene 3ˊ-untranslated regions in enhancing gene expression in transgenic riceseed. Transgenic Res (2012 ) 21:545–553.) The DNA fragment between the recognition sequences of SmaI and SacI was replaced with the DNA fragment shown in Sequence 2 to obtain a recombinant vector, which was named pGPTV-SPAA, and pGPTV-SPAA...

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Abstract

The invention discloses a preparation method of a blood-pressure-lowering rice material, an adopted gene and an adopted protein. The preparation method of the blood-pressure-lowering rice material comprises the step that the expression quantity of the blood-pressure-lowering related protein in a target plant is increased to obtain a plant with a function of lowering the blood pressure of animals,wherein the blood-pressure-lowering related protein is obtained by directly connecting n kinds of angiotensin converting enzyme inhibitory peptides or connecting the angiotensin converting enzyme inhibitory peptides through connecting peptides, n is a natural number greater than or equal to 1, the blood-pressure-lowering related protein contains m angiotensin converting enzyme inhibitory peptides,m is a natural number greater than or equal to 2, and a recognition sequence for proteases is contained between every two angiotensin converting enzyme inhibitory peptides of the blood-pressure-lowering related protein. It is proved through experiments that the blood-pressure-lowering rice material can effectively lower the blood pressure of animals with high blood pressure.

Description

technical field [0001] The invention relates to a method for preparing blood pressure-lowering rice materials and used genes and proteins in the field of plant genetic engineering. Background technique [0002] Hypertension is a common cardiovascular disease, which can cause damage to the brain, kidneys and cardiovascular system, and is an important factor causing stroke, coronary heart disease and heart failure. Angiotensin converting enzyme (Angiotensin converting enzyme, ACE) plays a key role in blood pressure regulation, it can convert inactive angiotensin I into active angiotensin II. Angiotensin II is the strongest vasoconstrictor in the body, and it can also stimulate the secretion of aldosterone, which directly causes blood pressure to rise. At the same time, angiotensin-converting enzyme can also degrade bradykinin, which has a vasodilator effect, and indirectly cause blood pressure to rise. Therefore, angiotensin-converting enzyme is the primary target for the pr...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H6/46C07K19/00C12N15/62
CPCC07K14/00C07K2319/00C12N15/8251
Inventor 曲乐庆钱丹丹
Owner INST OF BOTANY CHINESE ACAD OF SCI