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Novel microorganism and production method for urolithins using same

A manufacturing method, urolithin technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of not producing urolithin, and achieve the effect of promoting mitophagy

Active Publication Date: 2019-10-11
DAICEL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the accumulation concentration of urolithin C in the fermentation broth is about 2mg / L, which is not suitable for the above purpose
In addition, it has been reported that although the Gordonibacter pamelaeae DSM 19378 strain, which is a close species of Gordonibacterurolithinfaciens, has the ability to produce urolithin, other intestinal bacteria such as Eggerthella lenta DSM2243 strain, Eggerthella sinensis DSM 16107 strain, and Paraeggerthella honkongensis DSM 16106 strain have the ability to produce urolithin. Bacteria do not have the ability to produce urolithin (Non-Patent Document 8)

Method used

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  • Novel microorganism and production method for urolithins using same
  • Novel microorganism and production method for urolithins using same
  • Novel microorganism and production method for urolithins using same

Examples

Experimental program
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Effect test

Embodiment 1

[0140] The isolation and taxonomic properties of Eggerthella sp. DC 3563 (NITE BP-02376) as the present strain are described below.

[0141] In order to isolate this strain, ellagic acid with a final concentration of 1 g / L was added to ABB medium (manufactured by OXOID Company), and then heat sterilized, and the gas phase was heated with N 2 :CO 2 :H 2 (80% / 10% / 10%) gas replacement was performed, and the resulting medium was used as a minimal medium. Human feces were inoculated into a minimal medium, and anaerobic culture was performed at 37°C. The cultivated culture solution was diluted and spread on ABB agar medium. Anaerobic culture was carried out at 37°C, and many single bacteria were obtained. The obtained plural single bacteria were inoculated into a minimal medium, respectively, and anaerobic culture was performed at 37°C.

[0142] Quantification of urolithins in the culture solution after culture was carried out by the following method.

[0143] Urolithins were ...

Embodiment 2

[0158] DNA was extracted from the DC 3563 strain as the present bacterial strain, and the full length of the 16S rRNA gene was amplified by PCR using universal primers specific for the 16S rRNA gene, and the base sequence of the 16S rRNA gene of the present bacterial strain was determined (as SEQ ID NO: 1).

[0159] When a homology search was performed using the International Base Sequence Database (DDBJ), the DC 3563 strain as the present strain had 99.9% homology with the Eggerthella lenta JCM 9979 (AB558167) strain.

[0160] In addition, after multiple alignment of the base sequences of each reference strain with high homology with this strain obtained from the nucleic acid database, a molecular phylogenetic tree was prepared by the NJ method. As a result, the DC 3563 strain as this strain It forms a cluster with species composed of the genus Eggerthella and appears as a closely related species.

[0161] As a result of comprehensive judgment of the above-mentioned method, ...

Embodiment 3

[0164] The DC 3563 strain, which is the present strain, was inoculated into the minimal medium described in Example 1, and cultured anaerobically at 37° C. for 2 weeks. After completion of the culture, quantitative analysis of urolithins was carried out by the method described in Example 1.

[0165] As a result, urolithin C was obtained at a molar yield of 90.3% relative to the added ellagic acid.

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Abstract

The present invention provides: a novel microorganism that has high urolithin-production capability; and a production method for urolithins that uses the novel microorganism. A microorganism that belongs to genus Eggerthella and produces urolithins.

Description

technical field [0001] The present invention relates to a novel microorganism and a method for producing urolithins using the microorganism. Background technique [0002] Urolithins represented by urolithin A and urolithin C are metabolites of ellagic acid derived from ellagitannins contained in pomegranate, raspberry, blackberry, cloudberry, strawberry, walnut, etc. be known. [0003] Ellagitannins are known to be classified as hydrolyzable tannins and, when ingested, are hydrolyzed in the body and converted to ellagic acid. In addition, it is also present in fruits etc. as ellagic acid. Such ellagitannins, ellagic acid, have very low intestinal absorption in vivo, but are known to be further metabolized by human colonic microflora and converted into urolithins when they are ingested. [0004] For example, regarding the production of urolithins in vivo, it has been reported that after ingesting ellagitannins such as geraniin (Geraniin) in rats, urolithins in urine were a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N1/20C12P7/22C12R1/01
CPCC12N1/20C12N15/09C12P17/06C12R2001/01C12N1/205
Inventor 工藤真丈中岛贤则山本浩明
Owner DAICEL CORP
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