A conductive polypyrrole/extracellular matrix composite film and its preparation method
A technology of composite thin film and external matrix, which is applied in the field of biomedicine, can solve the problems of cytotoxicity, apoptosis or immune response, etc., and achieve the effect of simple process, easy realization and good electrical conductivity
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Embodiment 1
[0024] (1). The fibroblasts previously cultured in the culture flask were detached. After centrifugation, they were suspended with high-sugar DMEM medium and counted with a counting plate at 5×10 4 piece / cm 2 The cell density of the cells was seeded in multi-well plates, and placed in a constant temperature of 37 ° C, 5% CO 2 Cultured in a cell culture box, the medium was changed once every 2 days, and the culture period was 7 days, and a complete cell sheet was finally formed;
[0025] (2). Treat the cell sheet with repeated freeze-thaw method, and freeze-dry to obtain extracellular matrix film; (3). Prepare 0.02mol L -1 The pyrrole monomer solution, stirred evenly;
[0026] (4). With FeCl 3 ·6H 2 O: The molar ratio of pyrrole monomer is 20:9 to prepare FeCl 3 Solution, add the hydrochloric acid solution that is 1:3 with the molar ratio of pyrrole monomer, stir evenly;
[0027] (5). Mix the above two solutions and stir for 1 min to form a polypyrrole polymerization solu...
Embodiment 2
[0031] (1). Detach the osteoblasts previously cultured in culture flasks. After centrifugation, suspend them with high-sugar DMEM medium and count them with a counting plate at 1×10 5 piece / cm 2 The cell density of the cells was seeded in multi-well plates, and placed in a constant temperature of 37 ° C, 5% CO 2 Cultivate in a cell incubator, change the medium once every 2 days, and the culture period is 5 days, and finally form a complete cell sheet;
[0032] (2). The cell sheet is treated with repeated freezing and thawing, and freeze-dried to obtain an extracellular matrix film;
[0033] (3). Prepare 0.01mol·L -1 The pyrrole monomer solution, stirred evenly;
[0034] (4). With FeCl 3 ·6H 2 O: The molar ratio of pyrrole monomer is 20:13 to prepare FeCl 3 Solution, add the hydrochloric acid solution that is 1:5 with the molar ratio of pyrrole monomer, stir evenly;
[0035] (5). The above two solutions were mixed and stirred for 10 minutes to form a polypyrrole polymeri...
Embodiment 3
[0038] (1). The fibroblasts previously cultured in the culture flask were detached. After centrifugation, they were suspended with high-sugar DMEM medium and counted with a counting plate at 1×10 6 piece / cm 2 The cell density of the cells was seeded in multi-well plates, and placed in a constant temperature of 37 ° C, 5% CO 2 Cultivate in a cell incubator, change the medium once every 3 days, and the culture period is 10 days, and finally form a complete cell sheet;
[0039] (2). The cell sheet is treated with repeated freezing and thawing, and freeze-dried to obtain an extracellular matrix film;
[0040](3). Prepare 0.2mol·L -1 The pyrrole monomer solution, stirred evenly;
[0041] (4). With FeCl 3 ·6H 2 O: The molar ratio of pyrrole monomer is 20:19 to prepare FeCl 3 Solution, add the hydrochloric acid solution that is 1:10 with the molar ratio of pyrrole monomer, stir evenly;
[0042] (5). The above two solutions were mixed and stirred for 5 minutes to form a polypyr...
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