Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of CBD as drug for treating METH addiction

A technology for addictive drugs and uses, applied in the direction of drug combinations, active ingredients of hydroxyl compounds, nervous system diseases, etc., can solve the problems of no effective drugs for drug addiction, drug abuse and unknown molecular mechanism of addiction, etc. achieve better therapeutic effects

Pending Publication Date: 2019-10-18
KUNMING MEDICAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Despite the rapid development of behavioral and pharmacological studies in the field of drug abuse research, the exact molecular mechanisms of drug abuse and addiction effects are still unknown, and there are currently no effective drugs for the treatment of drug addiction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of CBD as drug for treating METH addiction
  • Application of CBD as drug for treating METH addiction
  • Application of CBD as drug for treating METH addiction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Establishment of a METH-dependent rat CPP model.

[0023] experiment method:

[0024] 70 male SD rats (180-220 g) were purchased from the Experimental Animal Center of Kunming Medical University. All rats were housed in a temperature-controlled room (23°C ± 1°C), rats could drink food and water freely, and after a 12-hour light / dark cycle, all rats were acclimated to the environment in the CPP device for 3 days before the experiment was performed .

Embodiment 2

[0025] Embodiment 2 carries out CPP experiment, detects the effect of CBD on METH inducing CPP

[0026] 1. Test method:

[0027] CBD was dissolved in physiological saline solution of 5% dimethyl sulfoxide and 5% Tween 80 and injected intraperitoneally at doses of 10, 20, 40, and 80 mg / kg. The effects of different concentrations of CBD (10, 20, 40, 80 mg / kg) on ​​METH-induced CPP were observed. Rats were randomly divided into 7 groups: control group (normal saline, 10ml / kg, ip), METH group (METH, 2mg / kg, ip), CBD group (CBD, 40mg / kg, ip), CBD (10mg / kg) kg)+METH group, CBD(20mg / kg)+METH group, CBD(40mg / kg)+METH group and CBD(80mg / kg)+METH group. Rats were intraperitoneally injected with CBD (10, 20, 40, 80 mg / kg) or saline for 1 h, and then intraperitoneally injected with METH (2 mg / kg) or saline (10 ml / kg).

[0028] CPP experimental protocols such as figure 1 As shown in (a), it consists of three stages:

[0029] On days 1-3, each rat was free to move between the black and...

Embodiment 3

[0035] The expression changes of Sigma1R, AKT, p-AKT, GSK-3β, p-GSK-3β, CERB and p-CREB in four brain regions were detected by WesternBlot.

[0036] 1. Test method

[0037] After killing the rats, the rat brain tissue was placed on ice, and the prefrontal cortex, hippocampus, nucleus accumbens and ventral tegmental area were isolated and stored at -80°C for later use. Brain tissue was lysed with enhanced RIPA protein lysis buffer containing 1% PMSF and protease inhibitors. The protein concentration was detected using the BCA protein assay kit. Equal amounts (25 μg) of protein were separated by 12% SDS-PAGE and transferred to 0.45 μm PVDF membranes. The PVDF membrane was blocked with 5% nonfat milk at room temperature for 2 hours, and the primary antibody (1:1000) was added overnight in a refrigerator at 4°C. Subsequently, secondary antibody (1:5 000) was added and incubated at room temperature for 1 h, the membrane was washed 10 min×4 times with TBST, developed with ECL chr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of cannabidiol as a Sigma1R / AKT / GSK-3beta / CREB signal path inhibitor and as a drug for treating METH addiction. It is found that METH can induce rat CPP, CBD has an inhibitory effect on the METH addiction and phosphorylation of an AKT-GSK3beta-CREB signal path, the CBD can weaken the phenomenon that the METH induces the rat CPP by regulating a Sigma1R / AKT / GSK-3beta / CREB signal path after being in use in advance, and the therapeutic effect of the high-dose CBD (40, 80 mg / kg) is better. The application of the CBD is conductive to knowing the pharmacological effects of the CBD, provides new ways to treat the METH addiction, provides new theoretical guidance for drug abuse mechanism research and withdrawal therapy, and also provides new ideas and options for developing effective drugs for treating drug addiction.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of CBD as a medicine for treating METH addiction, and the effect of CBD as a Sigma1R / AKT / GSK-3β / CREB signaling pathway inhibitor in reducing methamphetamine-induced CPP in rats. Background technique [0002] Methamphetamine (METH) belongs to amphetamine stimulants, is a highly dependent psychoactive substance, is the most popular new synthetic drug in today's society, the appearance is white transparent irregular crystals, commonly known as "ice", its It has the characteristics of strong mental dependence, high relapse rate and high neurotoxicity. Long-term use can have significant toxic effects on the nervous system and cause serious physical and psychological damage to the abuser. Numerous studies have shown that the dopamine system, activation of microglia, neuronal apoptosis, and autophagy are involved in METH-induced neurotoxicity. But so far, the mechanism of METH-induced ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P25/36
CPCA61K31/05A61P25/36
Inventor 曾晓锋张瑞林张冬先杨根梦刘柳李娟黄俭李媛媛沈宝玉
Owner KUNMING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com