A kind of preparation method of gardenia fermented bacterium
A technology for fermenting bacteria and fermented soy bean, which is applied in the field of preparation of two-way solid fermentation products of traditional Chinese medicine, can solve the problems of reduced enzyme activity and destruction of β-glucosidase, etc., so as to achieve process and quality improvement, change dosage form, and improve antidepressant effect. Effect
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specific Embodiment approach 1
[0014] Specific embodiment one: the preparation method of fermented fungus of gardenia jasminoides in this embodiment is characterized in that the preparation method is carried out according to the following steps:
[0015] 1. Add water to decoct Gardenia, Artemisia annua, and mulberry leaves, then soak soybeans with the decoction liquid, and then sterilize the soybeans at high temperature after absorbing the decoction liquid to expand; to obtain the fermentation substrate;
[0016] 2. The fermentation substrate prepared in step 1 is inoculated with microorganisms containing β-glucosidase and protease, mixed and fermented, and the fermented bacteria of Gardenia jasminum is obtained;
[0017] Among them, the dry weight ratio of soybean, gardenia, Artemisia annua and mulberry leaves is 100:(20-40):(5-10):(5-10).
specific Embodiment approach 2
[0018] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: the preparation method, which is characterized in that the microorganisms described in step 2 are Bacillus subtilis and / or Amanita agaricus. Others are the same as the first embodiment.
specific Embodiment approach 3
[0019] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: in step 2, bacillus subtilis (10 5 ~10 6 CFU / ml) and / or Pyrrhimus umbilifolii (10 5 ~10 6 spores / ml) inoculum volume is 20-40ml / kg. Others are the same as the first or second embodiment.
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