High-performance aniline-degrading bacterium and application thereof in aniline wastewater treatment
An aniline-degrading bacteria and high-efficiency technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, sustainable biological treatment, etc., can solve problems such as unsatisfactory economy, high equipment requirements, and short reaction time , to achieve strong practical value, high degradation efficiency, and improved degradation efficiency
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Embodiment 1
[0034] Embodiment 1: Isolation, screening and identification of bacterial strains
[0035]The high-efficiency aniline-degrading bacteria AD1 provided in this example is obtained by separating and screening from the activated sludge in the stable operation period of the laboratory self-controlled SBR reactor. The effective volume of the self-controlled SBR reactor is 1.5L, and the operation cycle is 8h, with three cycles per day. , Each cycle is divided into 5 stages: water intake, aeration, sedimentation, drainage, and standing. The feed water of the reactor is artificially prepared, and its composition is an inorganic salt medium with 200 mg / L aniline as a carbon source. The specific separation and screening methods are as follows:
[0036] 1) Isolation and purification of strains
[0037] Put 10mL of activated sludge obtained from a self-controlled SBR reactor containing 200mg / L aniline into an Erlenmeyer flask filled with 90mL of sterile water, put several glass beads in i...
Embodiment 2
[0055] Embodiment 2: Degradation effect experiment under different aniline concentrations
[0056] In this example, the aniline degradation effect of the high-efficiency aniline-degrading bacteria AD1 screened in Example 1 was tested at different aniline concentrations. In the inorganic salt medium with the concentration of 400, 600, 800, 1000 and 1200 mg / L respectively, shake culture in a shaker at a temperature of 28 °C and a speed of 180 r / min for 72 hours, and measure the OD at 72 hours 600 and residual aniline concentration.
[0057] Measurement results such as image 3 As can be seen from the figure, the aniline degradation rate and OD 600 The values show a gradually decreasing trend. When the aniline concentration is 400mg / L, the aniline degradation rate of 72h is 100%, OD 600 Value is 0.552; when the aniline concentration is 600mg / L, the aniline degradation rate of 72h is 71%, OD 600 Value is 0.526; when the aniline concentration is 1200mg / L, the aniline degrada...
Embodiment 3
[0058] Embodiment 3: Degradation effect experiment under different pH values
[0059] In this example, the aniline degradation effect of the high-efficiency aniline-degrading bacteria AD1 screened in Example 1 is tested at different pH values. The specific steps are: inoculate the AD1 seed solution cultivated to the logarithmic growth phase with 5% (V / V) Inoculate the appropriate amount of the solution into inorganic salt medium with pH values of 5.0, 6.0, 7.0, 8.0, and 9.0 and aniline concentration of 600 mg / L, shake and culture for 72 hours in a shaker at a temperature of 28 ° C and a speed of 180 r / min, and measure the time of 72 hours. The growth condition of the strain OD 600 and residual aniline concentration.
[0060] Measurement results such as Figure 4 It can be seen from the figure that the strain AD1 grows best when the pH is 7.0, and the OD at this time 600 The value is 0.806; the degradation effect of the strain on aniline is also the best when the pH is 7.0...
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