Tissue culture and rapid propagation method of impatiens hawkeri

A technology of impatiens group and impatiens, which is applied in the field of plant cultivation, can solve the problems of large amount of material required, low rooting rate, which is not conducive to the rapid and mass reproduction of impatiens in New Guinea, and achieves high induction rate, high differentiation ability, The effect of fast and mass reproduction

Inactive Publication Date: 2019-10-29
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the rooting rate is low when cutting, and the amount of material required is large, the cost is high, and the flowering period is difficult to be uniform
Therefore, the above two methods are not conducive to the rapid and large-scale reproduction of New Guinea impatiens
[0004] There have been reports on tissue culture of Impatiens New Guinea, but the induction rate of adventitious buds induced by explants of existing tissue culture is low

Method used

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  • Tissue culture and rapid propagation method of impatiens hawkeri
  • Tissue culture and rapid propagation method of impatiens hawkeri
  • Tissue culture and rapid propagation method of impatiens hawkeri

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A New Guinea Impatiens tissue culture rapid propagation method, the specific steps comprising: seed sterilization, explant induction of adventitious buds, adventitious bud proliferation culture and rooting culture;

[0033] a. Seed sterilization: Wrap the New Guinea Impatiens seeds with a single layer of gauze, rinse them with running water for 30 minutes, sterilize them with 70% alcohol on the ultra-clean workbench for 10 seconds, wash them twice with sterile water, and rinse them with sterile water Occasionally shake the glass bottle for 1-2 minutes, sterilize the New Guinea impatiens seeds in 2% sodium hypochlorite solution for 8 minutes, shake the glass bottle containing the sterilizing agent continuously during the sterilization period, and then wash it with sterile water for 4-5 times , shake the glass bottle for 1-2 minutes when washing with sterile water, blot the moisture on the surface of the seeds with sterilized filter paper, inoculate them in MS medium, trea...

Embodiment 2-6

[0047] Embodiment 2-6: all the other conditions are the same, only the sterilization time in the sodium hypochlorite solution is different, implement

[0048] Example 2-6 The sterilization time in sodium hypochlorite solution is 10, 12, 14, 16 and 18 minutes.

[0049] The experimental results are as follows:

[0050] It can be seen from the above table that with the increase of the sterilization time using 2% sodium hypochlorite solution, the contamination rate of seeds decreases, and the germination rate of seeds also decreases thereupon. But use 2% sodium hypochlorite solution to sterilize the seeds within 12min ( figure 1 a4.) Although the pollution rate is high, the aseptic seedlings grow vigorously, the plants are bright green, the cotyledons are fully expanded, the hypocotyls are strong and erect, and the roots are many and long; when the sterilization time reaches 14 minutes ( figure 1 a5.), the growth of sterile seedlings is deformed, it is light yellow to light gree...

Embodiment 7-14

[0053] A New Guinea Impatiens tissue culture rapid propagation method, the specific steps comprising: seed sterilization, explant induction of adventitious buds, adventitious bud proliferation culture and rooting culture;

[0054] a. Seed sterilization: Wrap the New Guinea Impatiens seeds with a single layer of gauze, rinse them with running water for 30 minutes, sterilize them with 70% alcohol on the ultra-clean workbench for 10 seconds, wash them twice with sterile water, and rinse them with sterile water Oscillate the glass bottle for 1-2 minutes, sterilize New Guinea Impatiens seeds in 2% sodium hypochlorite solution for 12 minutes, shake the glass bottle containing the sterilizing agent continuously during the sterilization period, and then wash it with sterile water for 4-5 times , shake the glass bottle for 1-2 minutes when washing with sterile water, blot the moisture on the surface of the seeds with sterilized filter paper, inoculate them in MS medium, treat 30 bottles...

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Abstract

The invention relates to the field of plant cultivation, in particular to a tissue culture and rapid propagation method of impatiens hawker. According to the method, aseptic seedlings are treated intocotyledonary nodes with hypocotyls and adaxial half-end cotyledons to serve as the optimal explants for inducing adventitious buds, so that the induction rate of the adventitious buds is 100%, the adventitious bud induction rate of the explants is high, 40.71 adventitious buds are induced averagely, and besides, more undifferentiated buds are obtained by induction and have higher differentiationcapacity; rapid and massive propagation of impatiens hawker is facilitated by seed sterilization, explant induction for the adventitious buds and multiplication culture and rooting culture of the adventitious buds.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for tissue culture and rapid propagation of Impatiens New Guinea. Background technique [0002] New Guinea Impatiens is a perennial evergreen herb. Stem fleshy, much branched. The leaves are alternate, sometimes the upper part is whorled, the leaves are ovate-lanceolate, and the veins are red. The flowers are solitary or several in corymbs, with long flower stalks and pink, pink, orange, purple and white petals. The flowering period is from June to August. It likes hot weather, requires sufficient sunlight and deep, fertile, well-drained soil. Afraid of cold, the whole plant will wither in case of frost. In the rainy season, poor drainage and poor ventilation are prone to powdery mildew or rotten roots and even fallen leaves. Pay attention to ventilation and lower the temperature. Sow and reproduce. Suitable for flower beds, flower border arrangements or potted pl...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 黄美娟黄海泉黄小云黄武略魏杰汪琼闻永慧鄢波
Owner SOUTHWEST FORESTRY UNIVERSITY
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