Near-infrared fluorescent probe targeting cyp1b1 enzyme and its preparation and use

Abstract

The present invention provides a near-infrared fluorescent probe targeting a CYP1B1 enzyme, and preparation and use thereof. The fluorescent probe comprises an affinity ligand, a signal group, and a linking chain for linking the ligand and the signal group. The linking chain comprises a plurality of ethylene glycol fragments. The affinity ligand is an alpha-naphthoflavone derivative, and the signal group is a near infrared fluorescent molecule. The near-infrared molecular probe targeting a tumor specific marker CYP1B1 enzyme effectively avoids the influence of introduction of the signal groupon inhibitory activity for the CYP1B1 enzyme, promotes application of the near-infrared molecular probe in the tumor in-vivo imaging, and has a good application prospect and clinical transformation value in the field of early diagnosis of tumor.

Description

technical field

[0001] The invention relates to the field of near-infrared in vivo imaging and molecular imaging probes, and relates to a near-infrared fluorescent probe targeting CYP1B1 enzymes and its preparation and use, specifically a near-infrared fluorescent dye and cytochrome P4501B1 The diagnostic reagent complex composed of ligands specifically bound by the enzyme can be specifically enriched in tumor cells by targeting the cytochrome P450 1B1 enzyme, and can be used for tumor-targeted fluorescence imaging and early diagnosis of tumors. Background technique

[0002] In the early diagnosis and localization of tumors, non-invasive molecular imaging techniques can not only display the location of tumors through specific probes, but also reflect the changes of specific signaling molecules in tumor cells. Generally, receptors or proteins specifically expressed in tumor cells can be used as biological targets for molecular imaging, and antibodies or small molecules with h...

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