A method for identifying human-derived cells and mouse-derived cells
A mouse and cell technology, applied in the field of cell identification, can solve the problems of obtaining results in about a day at the fastest, complex identification process, and high cost, achieving low loss cost, enhanced identification frequency, and reduced time cost. Effect
Active Publication Date: 2022-01-11
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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Problems solved by technology
The STR identification method requires the use of high-end instruments, which are difficult to implement in general laboratories; the identification process is complex, requiring a variety of instruments, reagents and consumables, and the cost is expensive (the identification price is about 2,000 yuan / sample); sample identification requires the fastest It takes about a day to get the results, which takes a long time
Method used
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Embodiment 1
[0023] An embodiment of the method for identifying human-derived cells and mouse-derived cells of the present invention, the method realizes the distinction based on fluorescence observation after the cells are labeled with a fluorescent dye, and specifically includes the following steps:
[0024] The source of sample cells is human and mouse, which can be in the form of tissue sections, cell lines and primary cells; fluorescent dyes include DAPI (Chinese name: 4',6-diamidino-2-phenylindole, English name: 4',6-diamidino-2-phenylindole) or other DNA-labeled fluorescent materials (such as: hoechst dye, etc.); the fluorescent dye was diluted to 10 μg / ml, and after incubating the cell sample for about 5-10 minutes, rinse with phosphate buffered saline (English name: phosphate buffersaline, referred to as PBS solution) for 5 to 10 minutes, and then observed and resolved under a fluorescent microscope.
[0025] The result is as Figure 1~3 as shown, figure 1 It is the fluorescence...
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The invention discloses a method for quickly identifying human-derived cells and mouse-derived cells, comprising the steps of: providing human-derived / mouse-derived identification samples, incubating with DAPI solution, cleaning the samples, and then observing under a fluorescent microscope Identification: The samples with DAPI accumulation spots in the nucleus are from mice, and the samples without DAPI accumulation spots in the nucleus are from human. The present invention has low loss cost in terms of reagents and materials, and the observation experiment can be carried out under a fluorescent microscope, and the cost is about 20 yuan / sample, which can be easily realized in ordinary laboratories; the identification operation process of the present invention is simple, and ordinary experimenters can It is easy to master the operation; the experimental results can be obtained within half an hour, which greatly reduces the time cost; this method makes up for the shortcomings of the existing technology, and can quickly perform human-derived samples and mouse-derived samples in ordinary laboratories. Identification, effectively enhance the identification frequency of laboratory samples, avoid cross-contamination, and reduce unnecessary losses.
Description
technical field [0001] The invention relates to the technical field of cell identification, in particular to a method for quickly identifying human-derived cells and mouse-derived cells. Background technique [0002] Biomedical research laboratories will use a large number of human-derived and mouse-derived experimental materials, including tissue materials, primary cells and cell lines, etc., and the identification of experimental materials is very necessary. According to statistics, about 30% of cell lines are cross-contaminated or misidentified. The use of such cells will lead to wrong research conclusions, irreproducible results, and failure of clinical cell therapy, which will waste a lot of time, energy and money. Cell short tandem repeat (short tandem repeat, STR) identification method is the representative of existing cell identification technology. [0003] The STR gene locus is composed of short tandem repeat sequences with a length of 3 to 7 base pairs, which is ...
Claims
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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 尹东王永强张寅
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV