Non-specific binding resisting polystyrene microspheres, preparation method therefor and storage method for non-specific binding resisting polystyrene microspheres

Abstract

The invention relates to non-specific binding resisting polystyrene microspheres, a preparation method therefor and a storage method for the non-specific binding resisting polystyrene microspheres. The preparation method comprises the steps: (1) sequentially adding epoxy polystyrene microspheres, glucan and a catalyst into a buffer solution, and carrying out a full reaction, so as to obtain a reaction solution; and (2) subjecting the reaction solution obtained in the step (1) to ultrasonic treatment firstly to achieve uniform mixing, then, carrying out heated refluxing, and carrying out centrifuge washing after heated refluxing is completed, thereby obtaining the non-specific binding resisting polystyrene microspheres. Surfaces of the non-specific binding resisting polystyrene microspheresprepared by the method disclosed by the invention are densely coated with glucan chains of different lengths, the quantity of remaining active groups is extremely small, and thus, the surface exposedarea of the polystyrene microspheres is reduced greatly; and compared with surface modification treated polystyrene microspheres prepared by the existing other methods, the adsorption capacity of thenon-specific binding resisting polystyrene microspheres to non-specific antibodies and active substances is remarkably lowered, so that the reliability of specific binding analysis signals is greatlyimproved.

Description

technical field [0001] The invention belongs to the technical field of chemical synthesis, and in particular relates to polystyrene microspheres resistant to non-specific binding, a preparation method and a storage method thereof. Background technique [0002] There are two main methods of coupling polystyrene microspheres and antibodies: one is to use chemical coupling agents to generate covalent bonds between functionalization (carboxyl / amino / aldehyde groups, etc.) and antibodies, and the other is to The method of physical adsorption relies on electrostatic force to make the antibody adsorb to the surface of polystyrene microspheres. However, neither of these two methods can avoid the chemical or physical adsorption of non-specific antibodies or other active substances on the surface of polystyrene microspheres. The adsorption of non-specific substances will greatly interfere with the downstream detection results, resulting in a decrease in the amount of antibody binding ...

AI Technical Summary

Problems solved by technology

The adsorption of non-specific substances will greatly interfere with the downstream detection results, resulting in a decrease in the amount of antibody binding on the surface of polystyrene microspheres, and untrue detection signals in the later stage.

[0003] Although the prior art has disclosed a method for obtaining polystyrene microspheres with low non-specific binding by modifying the surface of polystyrene microspheres, the current method has relatively high requirements for process conditions, and surface treatment The effect is still not particularly ideal, so that there are more residual active groups and exposed areas on the surface of polystyrene microspheres, resulting in non-specific adsorption of proteins and other substances, thus affecting the detection signal of specific binding antibodies