A biostorage system, construction method and application suitable for Synechococcus

A construction method, the technology of Synechococcus, applied in the field of biological storage system, can solve the problems of strain growth influence, strain death, long time, etc.
CN110438053BActive Publication Date: 2022-03-11TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2022-03-11

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Abstract

The invention discloses a biological storage system suitable for Synechococcus 7942, a construction method and an application. The promoter PpsbA2 was obtained from algae 6803; the terminator Trbcl was amplified from the integrated plasmid pBA3031; the toxic gene sepT2 was expressed with the iron deficiency inducible promoter PisiAB using pBR322 as the vector, and the transcription was terminated with the terminator Trbcl; the expression was expressed with the promoter PpsbA2 The antiviral gene sepA2 was constructed and transformed into Synechococcus sp. The system of the present invention grows normally without induction, and dies rapidly after induction. This has important theoretical and practical significance for the development and optimization of the biostorage system of Synechococcus, and also provides a reference for solving the biosafety problems of other cyanobacteria.
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Description

Technical field

[0001] The present invention belongs to the field of biological safety, and in particular to a biological storage system suitable for polyolis 7942 and polyolite 2973, a method and application of a construction method, which can be used to prevent the escape of the genetic engineering. Background technique

[0002] With the increasing depletion of traditional energy, the greenhouse effect is gradually serious, the environment is deteriorated, and the biological energy is increasingly received by its green environmental protection, and the development and utilization of replacement new energy is imminent. As a photosynthetic blue bacterium, polyol is directly utilized to produce biofuels and fine chemicals, which have been widely used as a new generation of "cytoplasma" in recent years. The polyolis 7942 is a commonly used model strain, which has clear genetic background, and the characteristics of polyolina 2973 have become a highly production potential due to its...

Claims

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