High resolution holographic microscope and method for living cell imaging

Abstract

The present invention discloses a high resolution holographic microscope and a method for living cell imaging, and belongs to the technical field of holographic microimaging. In a holographic opticalpath, when a secondary point light source passes through a living cell sample, diffracted object light interferes with undiffracted reference light to form an in-line hologram; the image is magnifiedby a high power microobjective, received by a CCD camera and stored in a computer; and a reconstructed image is obtained by computer numerical reconstruction. The invention uses a low power microobjective to convert a parallel light source into the secondary point light source combined with the object light and the reference light in the same direction during a recording process, makes full use ofa limited spatial bandwidth of the CCD camera, greatly improves the resolution of the holographic microscope system, uses a three-dimensional micron-scale displacement platform to precisely adjust the microscopic resolution and receives more interference outer ring fringes by shortening the recording distance, which includes high-frequency information of the sample playing a key role in reconstructing the image. Combined with the high power microobjective in front of the CCD camera, non-contact, non-invasive, fast, quantitative and high-resolution holographic microimaging of the living cell sample can be realized.

Description

technical field [0001] The invention relates to the technical field of holographic microscopic imaging, in particular to a high-resolution holographic microscope and method for living cell imaging. Background technique [0002] When a traditional optical microscope measures a sample, since the detector can only record the intensity information of the light wave of the object, it can only obtain the two-dimensional shape of the object. Electron microscopes and scanning probe microscopes require pretreatment of the sample to be tested before measurement, and the scanning process takes a certain amount of time, so it is impossible to perform non-contact, non-damaging, fast, and real-time measurement of the sample. The digital holographic microscopic imaging technology developed in recent years has the unique advantages of non-contact, non-destructive, fast, real-time and quantitative imaging of biological samples, which overcomes the limitations of traditional microscopic imagi...

AI Technical Summary

Problems solved by technology

[0005] Also widely used in off-axis digital holographic microscopes, the optical path of the system has the disadvantages of complex structure, high coherence requirements of the light source, many rear-end optical components, and difficult adjustment; and the separation of object light and reference light increases the density of interference fringes, without Make full use of the limited spatial bandwidth of CCD cameras [1-2] , so the system resolution is difficult to be further improved; and the distance between the sample and the recording surface in the optical path is difficult to get very close, which is not conducive to recording the high-frequency information of the sample.

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