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Ionic liquid/MCNT-modified electrode for sensitively detecting dopamine and preparation method and application of ionic liquid/MCNT-modified electrode

A technology of ionic liquids and modified electrodes, which is applied in the direction of measuring devices, electrochemical variables of materials, and material analysis through electromagnetic means, to achieve the effects of improving electrode detection performance, increasing electron transfer rate, and strong selectivity

Inactive Publication Date: 2019-11-15
HUIZHOU LEADAO ELECTRONICS MATERIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problem of accurately and sensitively detecting dopamine in the mixed solution of ascorbic acid, uric acid and dopamine, and provide a kind of ionic liquid / MCNT modified electrode sensitively detecting dopamine

Method used

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  • Ionic liquid/MCNT-modified electrode for sensitively detecting dopamine and preparation method and application of ionic liquid/MCNT-modified electrode
  • Ionic liquid/MCNT-modified electrode for sensitively detecting dopamine and preparation method and application of ionic liquid/MCNT-modified electrode
  • Ionic liquid/MCNT-modified electrode for sensitively detecting dopamine and preparation method and application of ionic liquid/MCNT-modified electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of ionic liquid / MCNT modified electrode:

[0038] (1) Preparation of chitosan solution: Dissolve an appropriate amount of chitosan in 1% HAc solution, ultrasonically dissolve to obtain a 3.5 mg / mL chitosan solution, and place it in a refrigerator for 12 hours;

[0039] (2) Preparation of MCNT / CS modification solution: Dissolve 3 mg MCNT in 1 mL chitosan solution, mix well, and oscillate ultrasonically for 30 min;

[0040] (3) Preparation of MCNT / CS / IL / GCE modified electrode: glassy carbon electrode 1.00, 0.30, 0.05μm Al respectively 2 o 3 Powder polished, then 1:1 HNO3 , absolute ethanol, and ultrapure water for 1 min each, and let it dry naturally for later use. Add a certain volume of IL to the MCNT / CS modification solution, the volume ratio of IL to MCNT / CS modification solution is 3:100, and mix with ultrasonic to obtain the MCNT / CS / IL modification solution. Take 5 μL of the modification solution and apply it on the polished surface. The surface of ...

Embodiment 2

[0043] Preparation of ionic liquid / MCNT modified electrode:

[0044] (1) Preparation of chitosan solution: Dissolve an appropriate amount of chitosan in 1% HAc solution, ultrasonically dissolve to obtain a 3.5 mg / mL chitosan solution, and place it in a refrigerator for 12 hours;

[0045] (2) Preparation of MCNT / CS modification solution: Dissolve 3 mg MCNT in 1 mL chitosan solution, mix well, and oscillate ultrasonically for 30 min;

[0046] (3) Preparation of MCNT / CS / IL / GCE modified electrode: glassy carbon electrode 1.00, 0.30, 0.05μm Al respectively 2 o 3 Powder polished, then 1:1 HNO 3 , absolute ethanol, and ultrapure water for 1 min each, and let it dry naturally for later use. Add a certain volume of IL to the MCNT / CS modification solution, the volume ratio of IL to MCNT / CS modification solution is 1:100 or 2:100 or 4:100 or 5:100, and ultrasonically mix to obtain MCNT / CS / IL modification 5 μL of the modified solution was applied onto the surface of a polished glass...

Embodiment 3

[0048] Ionic liquid / MCNT modified electrode for detection of dopamine:

[0049] 1. Performance testing of electrode modification layer

[0050] The MCNT / CS / IL / GCE modified electrodes prepared in Example 1 and Example 2 were respectively placed in a citric acid-disodium hydrogen phosphate (pH 7.0) solution containing 1.0 mM dopamine, and cyclic voltammetry was performed, and the scanning speed was 50mV / s, the test results are attached Figure 5 . As shown in the figure, with the increase of IL content in the modified electrode, the peak current of dopamine response on the modified electrode also increases, reaching the maximum when the IL content is 3%. When the content of IL exceeds 3%, because excess IL may cause the instability of the modified electrode, the response peak current of dopamine on the modified electrode begins to decrease, so the optimal volume of IL and MCNT / CS modified solution in the present invention can be determined The ratio is 3:100.

[0051] 2. Det...

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Abstract

The invention provides an ionic liquid / MCNT-modified electrode (MCNT / CS / IL / GCE-modified electrode) for sensitively detecting dopamine and a preparation method and application of the ionic liquid / MCNT-modified electrode. The MCNT / CS / IL / GCE-modified electrode is constructed by using a multiwalled carbon nanotube, chitosan and an ionic liquid, so that the overpotential can be reduced, the mass transfer rate of the dopamine is increased, and the functions, such as selective enrichment and permeation of analyte can be detected by effectively utilizing a special modified surface, thereby improving the selectivity and sensitivity of analysis. Due to the catalytic action of IL and MCNT, the modified electrode has the advantages that the interference of ascorbic acid and uric acid to detection of the dopamine can be relatively well eliminated and selectively sensitive detection on the dopamine in a mixed liquid in which the ascorbic acid, the uric acid and the dopamine coexist is achieved.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and relates to a preparation method of an ionic liquid / MCNT modified electrode and its application in dopamine detection. Background technique [0002] Dopamine is a very important neurotransmitter in the central nervous system of mammals and humans. Its content is relatively high in the caudate nucleus, about 50nmol / g, and its content in the extracellular fluid of the caudate nucleus is very low. Only 0.01-1μM. Dysregulation of dopamine neurons in the brain is an important cause of schizophrenia and Parkinson's disease. In addition, dopamine is an adrenaline-mimetic drug, which has the function of exciting the heart and increasing renal blood flow, and is used for hemorrhagic, cardiogenic and septic shock. Therefore, the research on the method of measuring dopamine is of great significance both in the study of physiological function and in clinical application. [0003] At present, the me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/327G01N27/3275G01N27/3277G01N27/3278G01N27/48
Inventor 奚亚男胡淑锦
Owner HUIZHOU LEADAO ELECTRONICS MATERIAL
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