Porcine epidemic diarrhea virus unmarked impedance type immunosensor and preparation method thereof

Abstract

The invention relates to a porcine epidemic diarrhea virus unmarked impedance type immunosensor and a preparation method thereof. The porcine epidemic diarrhea virus unmarked impedance type immunosensor is prepared by fixing monoclonal antibody PEDV-2C11 of the porcine epidemic diarrhea virus on an electrode surface modified by nanogold / molybdenum disulfide / reduced graphene composite nanometer material. The immunosensor is prepared by synthesizing the nanogold / molybdenum / reduced graphene (AuNPs / MoS2 / rGO) composite material, the sensor is simpler in preparation process, and meanwhile, the composite material is constructed through the nanogold and molybdenum disulfide / reduced graphene in advance, and the content of the nanogold in the composite material can be maintained at the stability; and meanwhile, the fixed amount of the antibody on the electrode surface is effectively increased since the adsorbed nanogold is possibly increased in the composite nanometer material, and the sensitivity of the immunosensor detection can be improved.

Description

technical field [0001] The invention belongs to the field of animal pathogen detection; in particular, it relates to a preparation method and application of a label-free impedance type immunosensor for porcine epidemic diarrhea virus. Background technique [0002] Porcine epidemic diarrhea (porcine epidemic diarrhea PED) is a contagious intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV), which is characterized by vomiting, diarrhea, dehydration, and depression. The disease was first reported in the UK in 1971, and the virus was first isolated in my country in 1980. Pigs of all ages can be infected with PEDV. Among them, suckling piglets, shelf pigs, and fattening pigs have the highest infection rate, and suckling piglets have the highest lethality rate. PED can occur throughout the year. Since 2011, new mutant strains have become popular in various places, and have now appeared in various countries and regions around the world. At present, PED h...

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Problems solved by technology

[0004] Chinese patent ZL201010295008.7 discloses a non-labeled impedance immunosensor for furaltadone residues, which is prepared by immobilizing AMOZ antibodies on the surface of gold electrodes through 1,4-benzenedithiol and nano-gold layer-by-layer assembly. Immunosensor, but the preparation process is relatively complicated, and the assembly time of nanogold needs to be strictly controlled to control the amount of adsorption on the electrode surface, so as to ensure the repeatability of the sensor

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