Application of quercetin in plant senescence promoter
A technology of plant aging and quercetin, which is applied in the direction of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of practical application that have not been reported, and achieve the advantages of easy-to-obtain raw materials, simple preparation process, and accelerated aging process Effect
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[0030] In a preferred embodiment, the preparation method of the quercetin plant aging accelerator comprises the following steps:
[0031] S1: Prepare quercetin mother liquor: weigh quercetin compound powder and dissolve it in a solvent to prepare quercetin mother liquor;
[0032] S2: Prepare 1 / 2 MS medium: weigh MS powder and dissolve it in distilled water, adjust the pH of the solution to 5.8-6.0, and prepare 1 / 2 MS medium;
[0033] S3: Prepare 1 / 2MS solution of 2-(N-morpholine)ethanesulfonic acid: Weigh 2-(N-morpholine)ethanesulfonic acid and add it to the 1 / 2MS medium to prepare 2-(N-morpholine)ethanesulfonic acid -morpholine) ethanesulfonic acid 1 / 2MS solution;
[0034] In the above steps, the reason for choosing 1 / 2MS solution to dissolve 2-(N-morpholine) ethanesulfonic acid is that this liquid medium can not only fully dissolve 2-(N-morpholine) ethanesulfonic acid, but also ensure the A certain osmotic pattern is formed to lay the foundation for quercetin to play its r...
Embodiment 1
[0044] The quercetin plant senescence accelerator comprises: the quercetin mother liquor whose concentration is 10mmol / L, the 2-(N-morpholine) ethanesulfonic acid 1 / 2MS solution that the concentration is 2.8mmol / L is mixed and made, 2-(N -Morpholine)ethanesulfonic acid 1 / 2MS solution is 2-(N-morpholine)ethanesulfonic acid dissolved in 1 / 2MS medium.
[0045] The specific preparation method is as follows:
[0046] S1. Prepare quercetin mother liquor: weigh quercetin powder, dissolve it in dimethyl sulfoxide, and prepare quercetin mother liquor with a concentration of 10 mmol / L for later use;
[0047] S2. Prepare 1 / 2 MS medium: weigh MS powder and dissolve it in distilled water, adjust the pH of the solution to 6.0, and prepare 1 / 2 MS medium;
[0048] S3. Configure 2-(N-morpholine)ethanesulfonic acid 1 / 2MS solution: weigh 2-(N-morpholine)ethanesulfonic acid and add it to the 1 / 2MS medium to obtain a concentration of 2.8mmol / L of 2-(N-morpholine)ethanesulfonic acid 1 / 2MS soluti...
Embodiment 2
[0051] The quercetin plant aging accelerator includes: a concentration of 8mmol / L quercetin mother solution, a concentration of 3mmol / L 2-(N-morpholine)ethanesulfonic acid 1 / 2MS solution mixed to make, 2-(N- Morpholine)ethanesulfonic acid 1 / 2MS solution is 2-(N-morpholine)ethanesulfonic acid dissolved in 1 / 2MS medium.
[0052] The specific preparation method is as follows:
[0053] S1. Prepare quercetin mother liquor: weigh quercetin powder, dissolve it in dimethyl sulfoxide, and prepare quercetin mother liquor with a concentration of 8 mmol / L for later use;
[0054] S2. Prepare 1 / 2 MS medium: weigh MS powder and dissolve it in distilled water, adjust the pH of the solution to 5.8, and prepare 1 / 2 MS medium;
[0055] S3. Configure 1 / 2MS solution of 2-(N-morpholine)ethanesulfonic acid: Weigh 2-(N-morpholine)ethanesulfonic acid and add it to the 1 / 2MS medium to obtain a concentration of 3mmol / 1 / 2MS solution of 2-(N-morpholine)ethanesulfonic acid in L;
[0056] S4. Configure ...
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