Antioxidant selenium polypeptide derived from pennsylvania bittercress herb and preparation method thereof

A technology of broken orchidna and anti-oxidation, which is applied in the biological field, can solve the problems of low selenium content and difficulty in supplementing selenium adequately, and achieve the effect of eliminating side effects

Active Publication Date: 2019-11-22
恩施德源硒材料工程科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an essential trace element for the human body, selenium has functions such as anti-oxidation and anti-fatigue. However, 72% of my country's areas are selenium-deficient areas, and 30% are severely selenium-deficien

Method used

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  • Antioxidant selenium polypeptide derived from pennsylvania bittercress herb and preparation method thereof
  • Antioxidant selenium polypeptide derived from pennsylvania bittercress herb and preparation method thereof
  • Antioxidant selenium polypeptide derived from pennsylvania bittercress herb and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Enzymolysis of Viola Leaf Broken Camelina

[0026] Dry and powder the whole plant of the broken rice chestnut, decolorize with absolute ethanol for 2 hours, centrifuge at 6400r / min for 15 minutes, air-dry the precipitate, add an alkaline solution with a pH of 8.5 according to a solid-liquid ratio of 1:40g / ml, and add alkaline protease to The crushed rice chestnut powder is subjected to enzymolysis at a temperature of 50°C, the enzymolysis time is 4h, and the enzyme-substrate ratio is 4000U / g, then acid protease is added at a temperature of 50°C, the enzymolysis time is 4h, and the enzyme-substrate ratio is 4000U / g. The proportion is 3000U / g. Inactivate the enzyme in a boiling water bath for 15 minutes, then rapidly cool down to room temperature, place in a centrifuge, centrifuge at 6400r / min for 15 minutes, take the supernatant and dry it, and obtain the crude extract of Selenium Peptide from Broken Camelina.

[0027] (2) Separation of enzymatic hydrolysis products...

Embodiment 2

[0050] The in vivo antioxidant activity of the natural antioxidant selenopolypeptide obtained in Example 1 was studied through a D-galactose-induced aging mouse model.

[0051] Select 60 ICR male mice and keep them in the breeding room with indoor humidity of 60±5% and ambient temperature of 23±2°C, with 12h alternating cycle of light and dark, during which the mice drink water and eat freely. The mice were fed adaptively for 7 days in the early stage, and then the mice were divided into 6 groups according to the random number table method: the aging model group, the blank control group, the selenoprotein group, the low-dose selenopolypeptide group of the broken rice chestnut, and the medium-dose group of the selenopolypeptide of the broken rice group, high-dose group of crushed orina selenopeptide. Dosage design basis: if the daily intake of adults (body weight 50kg) is 100 μg (calculated as selenium), the daily intake of mice in the middle dose group is 20 μg / kg BW, which is...

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Abstract

An antioxidant selenium polypeptide derived from pennsylvania bittercress herb is characterized in that the amino acid sequence is Gly-Arg-Val-Gly-Ser-Ser-Ser-Ser-SeCys, wherein SeCys is selenocysteine. A method for preparing the antioxidant selenium polypeptide derived from pennsylvania bittercress herb includes the steps of powdering, enzymolysis, ultrafiltration separation, ion exchange chromatography separation and RP-HPLC separation. The idea and method of extraction and application of conventional antioxidants and selenium supplements are changed, and the side effects possibly caused byartificially synthesized antioxidants are eliminated. The extracted antioxidant selenium polypeptide of pennsylvania bittercress herb is a natural antioxidant and a natural selenium supplement, and can replace a traditional synthesized antioxidant and has the effect of selenium supplement at the same time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an anti-oxidation selenium polypeptide derived from broken catalina and a preparation method thereof. Background technique [0002] Polypeptides are protein fragments composed of amino acids arranged in a certain order and have certain physiological functions. In human life activities, the digestion and absorption of peptides in the body is better than that of free amino acids. Some short peptides can prevent and treat diseases and regulate human body functions while providing nutrients necessary for human growth and development. These biologically active peptides are called bioactive peptides. Studies have found that many peptides from different sources have antioxidant capacity, such as soybean peptides and corn peptides. As an essential trace element for the human body, selenium has functions such as anti-oxidation and anti-fatigue. However, 72% of my country's areas are selenium-...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18A23L33/18
CPCC07K7/06C12P21/06A23L33/18A23V2002/00A23V2200/30A23V2250/55
Inventor 于添朱松杜朝东丛欣
Owner 恩施德源硒材料工程科技有限公司
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