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Application of TNFRSF13B gene rs34562254 SNP

A gene and genome technology, applied in the application field of the rs34562254 SNP of the TNFRSF13B gene, can solve the problems of unresearched, inconsistent, and not necessarily consistent with reasoning

Active Publication Date: 2019-11-22
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] TNFSF13 / TNFRSF13B plays an important role in host immune regulation, but when HCV invades the body, whether the gene polymorphism of TNFSF13 / TNFRSF13B will affect the outcome of HCV infection has not been studied at home and abroad, and there is no TNFSF13 or TNFRSF13B at home and abroad. Research reports on the correlation between SNPs and HCV infection
[0011] In epidemiological research, the determination of disease risk factors and correlation research can only be obtained through rigorous epidemiological design and research, and rigorous data analysis. All kinds of reasoning are usually the results and conclusions that are in line with scientific research hypotheses. However, the verified results may not conform to the reasoning, and sometimes even contradict

Method used

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  • Application of TNFRSF13B gene rs34562254 SNP
  • Application of TNFRSF13B gene rs34562254 SNP
  • Application of TNFRSF13B gene rs34562254 SNP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Preparation of substances for detecting human TNFRSF13B gene rs34562254 SNP / allele / genotype

[0068] A method for detecting TNFRSF13B gene rs34562254SNP / allele / genotype material in the human genome for detecting or screening the genetic susceptibility of hepatitis C in Chinese Han population, comprising the following steps:

[0069] S1: Extraction of Genomic DNA

[0070] (1) Purple hat vacuum blood collection tubes were used to collect the peripheral venous blood of the research subjects, absorb the whole blood, add an equal volume of phosphate buffer, and mix well; Wash with buffer solution, centrifuge to discard supernatant, and freeze;

[0071] (2) The leukocytes were resuspended with an equal volume of phosphate buffer, and then the DNA was extracted with a commercial QIAGEN DNA extraction kit, and the DNA solution was obtained for freezing;

[0072] (3) Get the DNA solution, measure the absorbance at 260nm and 280nm with UV spectrophotometry, OD260=1~20...

Embodiment 2

[0107] Example 2 Application of TNFRSF13B gene rs34562254 SNP / allele / genotype

[0108] The method for detecting the TNFRSF13B gene rs34562254 single nucleotide polymorphism or genotype in the human genome comprises the steps of: distributing the material for detecting the TNFRSF13B gene rs34562254 SNP or genotype in the human genome to 8 sterilized PCR tubes; taking 5 μl Add the system solution per well into a 384-well plate, and then add 1 μl / well DNA sample; set 2 negative controls in sterile double-distilled water and 2 positive controls in each plate to control cross-contamination; use a special membrane to separate the 384 wells loaded with samples The plate is sealed and centrifuged; placed in a fluorescent quantitative PCR instrument, and the PCR reaction conditions are set; after the PCR reaction is completed, the genotyping results are read.

[0109] Further, the detailed steps are as follows:

[0110] 1. SNP typing

[0111] The substance obtained in Example 1 for d...

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Abstract

The invention discloses a TNFRSF13B gene rs34562254 single nucleotide polymorphism (SNP) or genotype of a human genome in preparing products for detecting or screening Chinese Han population hepatitisC genetic susceptibility or products of the SNP related to the hepatitis C. in practical application, a substance detecting polymorphism / allele / genotype of rs34562254 can be combined with other substances (such as substances detecting other SNP / allele / genotype related to the hepatitis C) to prepare the products for screening Chinese Han population hepatitis C genetic susceptible people.

Description

[0001] The invention relates to the field of biomedicine, in particular to a rs34562254 single nucleotide polymorphism (single nucleotide polymorphism, SNP) in the human genome tumor necrosis factor receptor superfamily member 13B (tumor necrosis factor receptor superfamily member 13B, TNFRSF13B) gene Application in preparation of products for detection of genetic susceptibility to hepatitis C in Chinese Han population. Background technique [0002] SNP is a single nucleotide variation that occurs at a specific position in the genome, including transition and transversion. Base conversion refers to the substitution between purines (adenine A, guanine G) or between pyrimidines (cytosine C, thymine T), and transversion refers to the substitution between purines and pyrimidines. SNPs may exist in both coding and non-coding regions of genes. There are two types of SNPs in the coding region: synonymous and nonsynonymous. SNPs with synonymous mutations do not affect the amino acid...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6858C12N15/11
CPCC12Q1/707C12Q1/6858C12Q2600/156C12Q2600/172C12Q2561/101C12Q2563/107C12Q2531/113Y02A50/30
Inventor 岳明葛志军张津玮孔练花刘源黄鹏田亭凡豪志巫晶晶符祖强张云
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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