Multi-functional micro-fluidic chip and detection method for screening bacterial biofilm inhibitor

Abstract

The invention relates to a micro-fluidic chip and detection method for screening a bacterial biofilm inhibitor. The chip consists of substrates for integrating a micro array electrode and a cover plate comprising micro channels, and specifically comprises functional units including sample introducing channels, fluorescent reagent sample introducing channels, biofilm culturing regions, biofilm andmedicine interaction regions, biofilm fluorescent observation regions and the like. Through the adoption of the micro-fluidic chip disclosed by the invention, multiple functions of bacterial biofilm culturing, biofilm fluorescent observation, biofilm impedance monitoring and the like can be realized, the micro-fluidic chip can be applied to biofilm formation process observation and monitoring, medicine and biofilm interaction process on-line in-situ monitoring, bacterial biofilm inhibitor screening and the like; and the multi-functional chip has the advantage of detecting the changes of the biofilm in an in-situ nondestructive real-time online manner, and can analyze the action situation of different medicines on different bacterial biofilms, and then the high-efficiency screening of the biofilm inhibitor is realized.

Description

technical field [0001] The invention belongs to the technical field of cell and bacteria detection, and in particular relates to a multifunctional microfluidic chip for screening bacterial biofilm inhibitors. Background technique [0002] A biofilm is a microbial community composed of bacteria and their own secreted extracellular polymers. Compared with planktonic bacteria, biofilm has strong drug resistance. Once formed, it is difficult to completely remove it, which poses a huge threat to the medical, food and other industries. Screening inhibitors that can prevent the formation of biofilm is the key to solving the biofilm problem. effective way. Screening experiments for biofilm inhibitors are usually carried out in microwell plates, and crystal violet staining is used to evaluate the effect of inhibitors. This method consumes a lot of reagents, the operation steps are cumbersome, and the biofilm staining process and elution process are affected by human factors, result...

AI Technical Summary

Problems solved by technology

Although the electrical impedance analysis method has been applied to the research of bacterial biofilm detection, there is no integrated chip and detection technology that integrates microfluidic chip analysis technology, online monitoring of biofilm status and screening of bacterial biofilm inhibitors. The earth limits the application of microfluidic chips in the study of inhibitor-biofilm interaction and inhibitor screening

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