A quantitative detection method for meat sample adulteration based on different animal-derived meat characteristic peptides

A quantitative detection method and animal-derived technology, applied in the field of proteomics, can solve the problems of not being able to crack down on illegal traders, harming the economic interests and health of consumers, and being unable to judge the severity of adulteration.
CN110531019BActive Publication Date: 2021-05-18NANJING AGRICULTURAL UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2021-05-18

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Abstract

In view of the technical problem that in the current detection standard system for meat, animal-derived detection methods can only be qualitative or semi-quantitative, and the severity of adulteration cannot be judged, the present invention provides quantitative determination based on high performance liquid chromatography tandem mass spectrometry A variety of methods for meat of animal origin. In order to achieve the purpose of the present invention, the present invention screens characteristic polypeptides of standard meat samples of chicken, duck, pork, beef and mutton, and applies the characteristic polypeptides to quantitatively detect adulteration of meat samples. The quantitative detection method of the present invention comprises: pretreatment of the meat sample to be tested, and detection of characteristic polypeptides in the meat sample by high performance liquid chromatography-mass spectrometry. The invention also provides a relatively general screening method for standard meat-like characteristic polypeptides. Provide effective technical means for the supervision of meat sample adulteration in the meat market.
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Description

technical field

[0001] The invention belongs to the technical field of proteomics, and relates to a method for quantitatively detecting adulteration of meat samples based on characteristic polypeptides of different animal-derived meats. Specifically, it is a method for determining the sources and contents of different species in mixed meat samples based on high-performance liquid chromatography-mass spectrometry, and a method for screening species-specific polypeptides by using liquid chromatography-mass spectrometry. Background technique

[0002] In recent decades, the proportion of meat products in residents' consumption has increased year by year, but it has also brought some illegal activities, such as using cheap pork, duck, chicken, and even sick and dead animal meat and expired meat as high-priced cattle, The mutton incident has been exposed from time to time, which is shocking.

[0003] According to relevant surveys, the adulteration of meat products is mainly manif...

Claims

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