Emasculation and artificial pollination method for blueberry breeding

A technology for artificial pollination and blueberries, applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems that restrict the development of blueberry hybrid breeding, low hybrid seed setting rate, low pollen vigor, etc., and optimize pollen storage Conditions, development and enrichment, and the effect of optimizing flowering age

Inactive Publication Date: 2019-12-06
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of hybrid breeding, artificial pollination is a very critical part, but there are problems such as low hybrid seed setting rate, low fruit setting rate, and low pollen vigor, which seriously restrict the development of blueberry hybrid breeding

Method used

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  • Emasculation and artificial pollination method for blueberry breeding
  • Emasculation and artificial pollination method for blueberry breeding
  • Emasculation and artificial pollination method for blueberry breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Pretreatment before pollen collection: select unopened rabbit-eye blueberry inflorescences, bag them to prevent pollination, select flowers that have opened for 4 days in the inflorescences, spray 5% sucrose solution to the flowers, and then bag them;

[0021] (2) Pollen collection and storage: On the 5th day after the flowering of Rabbiteye blueberry, lightly touch the flower with tweezers, scatter the pollen in a glass bottle, dry it with a silica gel desiccant, store it in a sealed container at 4°C, and take out the pollen 2 hours before pollination.

[0022] (3) Castration and pollination: the cultivation temperature of highbush blueberry inflorescences is 20°C, select the inflorescences that are about to open on the female parent tree, remove the flower buds that have opened and are too small in the inflorescences, select the flower buds that are about to open, and remove the petals and stamens with tweezers , retain the stigma, bagged to prevent pollination, 4 ...

Embodiment 2

[0026] (1) Pretreatment before pollen collection: select unopened rabbit-eye blueberry inflorescences, bag them to prevent pollination, select flowers that have opened for 3 days in the inflorescences, spray the flowers with 1% sucrose solution, and then bag them;

[0027] (2) Pollen collection and storage: On the 4th day after the flowering of Rabbiteye blueberry, lightly touch the flower with tweezers, scatter the pollen in the glass bottle, dry it with a silica gel desiccant, store it in a sealed container at 4°C, and take out the pollen 1 hour before pollination.

[0028] (3) Castration and pollination: the cultivation temperature of highbush blueberry inflorescences is 15°C, select the inflorescences that are about to open on the female parent tree, remove the flower buds that have opened and are too small in the inflorescences, select the flower buds that are about to open, and remove the petals and stamens with tweezers , retain the stigma, bag the inflorescences to prev...

Embodiment 3

[0032] (1) Pretreatment before pollen collection: select unopened rabbit-eye blueberry inflorescences, bag them to prevent pollination, select flowers that have opened for 5 days in the inflorescences, spray 10% sucrose solution to the flowers, and then bag them;

[0033] (2) Pollen collection and storage: On the 6th day after the blooming of Rabbiteye blueberry flowers, lightly touch the flowers with tweezers, scatter the pollen in the glass bottle, dry it with a silica gel desiccant, store it in a sealed container at 6°C, and take out the pollen 3 hours before pollination.

[0034] (3) Castration and pollination: the cultivation temperature of highbush blueberry inflorescences is 30°C, select the inflorescences that are about to open on the female parent tree, remove the flower buds that have opened and are too small in the inflorescences, select the flower buds that are about to open, and remove the petals and stamens with tweezers , keep the stigma, bag the inflorescences t...

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Abstract

The invention discloses an emasculation and artificial pollination method for blueberry breeding. The method comprises the following steps of pretreatment before pollen collection, pollen collection and storage, and emasculation and pollination. Compared with the prior art, the method has the advantages that by spraying a sucrose solution onto flowers, optimizing pollen storage conditions, optimizing the flower age, optimizing pollination environment temperature and the like, the pollen vitality is improved, and the hybrid maturing rate and the fruit setting rate are increased.

Description

technical field [0001] The invention relates to the technical field of artificial breeding, in particular to a method for artificial pollination of blueberry breeding and emasculation. Background technique [0002] Blueberry is a perennial deciduous or evergreen shrub or small shrub species of the Rhododendronaceae Vaccinium genus, and is a new emerging fruit tree with high economic value. The fruit is edible, rich in anthocyanins, low in fat, low in sugar, and strong in antioxidant capacity. It is of great value in nutrition, health care, and medicine. With people's awareness of the nutritional value and health value of blueberry, its market demand is increasing day by day. [0003] Blueberry cultivars are mainly obtained through wild selection, seed selection, hybrid breeding and ploidy breeding, among which hybrid breeding is the most important method. In the process of hybrid breeding, artificial pollination is a very critical part, but there are problems such as low h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G7/06
CPCA01H1/02A01G7/06
Inventor 王平红聂飞文光忠文光琴李隆进廖优江
Owner GUIZHOU INST OF BIOLOGY
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