Check patentability & draft patents in minutes with Patsnap Eureka AI!

Application of limonin to preparation of drugs for preventing and treating acute kidney injury

A technology of acute kidney injury and limonin, which is applied in the field of medicine, achieves the effects of less toxic side effects, obvious renal protection, and promoting the proliferation of renal tubular epithelial cells

Active Publication Date: 2019-12-06
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on limonin in the field of acute kidney injury treatment has not been reported at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of limonin to preparation of drugs for preventing and treating acute kidney injury
  • Application of limonin to preparation of drugs for preventing and treating acute kidney injury
  • Application of limonin to preparation of drugs for preventing and treating acute kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: The protective effect of limonin on acute kidney injury caused by bilateral ischemia-reperfusion (BIRI) injury in mouse kidney

[0021] 1. Experimental animals: C57 mice, male, 8 weeks old, weighing 20-22g, SPF grade. 24 mice were randomly divided into 4 groups, 4 in the sham operation group, 6 in the operation group, 11 in the low dose (40mg / kg / d) treatment group, and 7 in the high dose (80mg / kg / d) treatment group.

[0022] Therapeutic drug: Limonin, which comes from the research group of Professor Liu Shijia of Nanjing University of Traditional Chinese Medicine. Its purification and preparation process has obtained an invention patent (patent number: CN201410005038.8). Limonin was dissolved with Kolliphor HS (Sigma).

[0023] 2. Treatment of each group

[0024] 1) Sham operation group: at room temperature, after anesthetizing mice with 3% sodium pentobarbital with 1ml / kg body weight, choose the median abdominal incision; after local disinfection, cut the ...

Embodiment 2

[0037] Example 2: Limonin can inhibit the apoptosis of renal tubular epithelial cells induced by hypoxia-reoxygenation in vitro

[0038] 1. Experimental materials:

[0039] Cells: human renal tubular epithelial cells (HK-2).

[0040] Medium: DMEM / F12 (1:1) medium containing 10% FBS.

[0041] Culture conditions: Conventional culture: 37°C with 5% CO 2 Incubator; anoxic culture: 37°C with 5% N 2 , 5% CO 2 incubator.

[0042] 2. Experimental treatment:

[0043] Human renal tubular epithelial cells HK-2 in good condition were mixed with 1.5×10 6 Seed in 6-well cell culture plate for overnight culture, replace the serum-free medium DMEM / F12 (1:1) for serum starvation for 24 hours, add limonin (10ng / mL) to the cell culture medium of the drug group for pretreatment for 1 hour , and then put the hypoxia group and drug group into the hypoxia incubator (5%N 2 , 5% CO 2 ) for 24 hours, and then transferred to a conventional incubator for reoxygenation for 2 hours. The protein w...

Embodiment 3

[0047] Example 3: Protective Effect of Limonin on Acute Kidney Injury Caused by Cisplatin Injection in Mice

[0048] 1. Experimental animals: C57 mice, male, 8 weeks old, weighing 20-22g, SPF grade. 35 mice were randomly divided into 3 groups, 9 in the control group, 14 in the operation group, and 12 in the treatment group (80mg / kg / d).

[0049] 2. Treatment of each group

[0050] 1) Control group: Mice were injected intraperitoneally with 20 mL / kg of normal saline, checked for marks, and placed in corresponding mouse cages.

[0051] 2) Cisplatin injection group: mice were intraperitoneally injected with the chemotherapy drug cisplatin 20 mg / kg to establish an acute kidney injury model. Verify the mark and place it in the corresponding mouse cage.

[0052] 3) Treatment group: the establishment of the cisplatin-induced acute kidney injury model was the same as above. Three days before the establishment, the mice were given intragastric administration of 80 mg / kg, once per day...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of medicine and relates to application of Chinese herbal medicine monomer limonin to preparation of drugs for preventing and treating acute kidney injury. Through study in vivo and in vitro, it is found that the limonin has the obvious biological effects of inhibiting apoptosis of renal tubular epithelial cells and promoting proliferation, and has the obvious renal protection effect on ischemia-reperfusion or chemotherapy drug cis-platinum induced acute kidney injury. Therefore, the limonin can be applied to preparation of the drugs for preventing and treating acute kidney injury, has the obvious kidney protection effect, and is small in toxic and side effect and low in cost.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the use of a Chinese herbal medicine monomer limonin, in particular to the use of limonin in the preparation of medicines for preventing and treating acute kidney injury. Background technique [0002] Acute kidney injury (Acute Kidney Injury, AKI) is a common clinical emergency and severe disease, with rapid onset, rapid progression and high mortality, which seriously threatens human health. The main manifestations of patients are the rapid decline of renal function and the accumulation of metabolic wastes. The clinical diagnosis is based on the increase of serum creatinine (Scr) and the decrease of urine output. The incidence of AKI is high and is increasing year by year. Epidemiological data show that even mild and reversible AKI can cause lasting damage to kidney tissue; severe AKI can lead to an irreversible decline in renal function and increase the risk of death. Due to the current l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/585A61P13/12
CPCA61K31/585A61P13/12
Inventor 傅海燕周栋刘史佳周先可卢嘉微
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com