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Single nucleotide polymorphisms (SNP) molecular marker located on porcine chromosome 1 and related to daily gain of pig and application of SNP molecular marker

A molecular marker and chromosome technology, applied in the field of molecular biotechnology and molecular markers, can solve the problems of restricting the application of molecular markers for complex traits, unable to confirm genes, and large span of candidate gene regions, so as to increase core competitiveness, increase gene frequency, The effect of speeding up the breeding process

Inactive Publication Date: 2019-12-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the candidate gene method has a certain degree of randomness in the selection of genes, and it is impossible to confirm whether the identified genes are the main effect genes.
However, the candidate gene region identified by the QTL mapping method has a large span and usually contains hundreds of genes, which greatly limits the application of molecular markers for complex traits in livestock genetics and breeding.

Method used

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  • Single nucleotide polymorphisms (SNP) molecular marker located on porcine chromosome 1 and related to daily gain of pig and application of SNP molecular marker
  • Single nucleotide polymorphisms (SNP) molecular marker located on porcine chromosome 1 and related to daily gain of pig and application of SNP molecular marker
  • Single nucleotide polymorphisms (SNP) molecular marker located on porcine chromosome 1 and related to daily gain of pig and application of SNP molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Experimental animals

[0035] The group of experimental pigs used in the present invention is 3916 purebred American Durocs from the breeding pig branch of Wen's Food Group Co., Ltd., which is the core group of the breeding pig branch, and the group pedigree records are detailed. In the breeding process of 3916 American Duroc pigs in the resource group selected in this experiment, the pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0036] 2. Sample collection

[0037] The above-mentioned piglet docked tail and ear tissues were collected, soaked in ethanol with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0038] 3. 50K SNP typing in the whole pig genome

[0039] The ear tissue or docked tail tissue collected from each individual of the 3916 Duroc breeding pigs selected from the above-mentioned resource groups, the whole genome DNA w...

Embodiment 2

[0049] Example 2 Target DNA sequence amplification and sequencing

[0050] (1) Primer design

[0051] The DNA sequence of SEQ ID NO: 1 on pig chromosome 1 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers.

[0052] The DNA sequences of the designed primers are as follows:

[0053] P001-F: 5'-CTGTGTCCCAAAGATCCCTGT-3',

[0054] P002-R: 5'-TGATGAGTGACCAGGCAGAG-3';

[0055] (2) PCR amplification

[0056] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0057] (3) DNA sequence determination

[0058] The identification of DNA sequence sequencing was ...

Embodiment 3

[0062] Example 3 SNP site g.87T>C effect analysis of molecular markers

[0063] The invention provides a SNP marker that can significantly increase the daily weight gain of Duroc pigs, and the SNP can be used for marker-assisted selection, which can greatly increase the breeding process of the lean meat rate of Duroc pigs. Through the assisted selection of molecular markers, if the present invention selects all the CC-type individuals with molecular markers that affect the daily weight gain traits of pigs into TT-type individuals, assuming that the pigs are bred to 160 days for slaughter, then each pig can gain 1.76kg more weight. According to the estimation of the slaughter rate of 50%, a large-scale pig farm with 10,000 heads can provide 8.8 tons more pork, which will bring huge economic benefits to the enterprise.

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Abstract

The invention relates to the fields of molecular biotechnologies and molecular marker technologies, in particular to a single nucleotide polymorphisms (SNP) molecular marker located on a porcine chromosome 1 and related to daily gain of a pig and application of the SNP molecular marker. The SNP site marked by the SNP molecular marker located on the porcine chromosome 1 and related to the daily gain of the pig corresponds to 162192627 T>C mutation on the chromosome 1 of an international porcine genome 11.1 version reference sequence. By optimizing a dominant allele of the SNP, the dominantallele frequency can be increased by generations, the daily gain of the pig is increased, pig genetic improvement development is accelerated, and thus economic benefits of pig breeding are effectivelyincreased.

Description

technical field [0001] The invention relates to the technical fields of molecular biotechnology and molecular markers, in particular to a SNP molecular marker located on pig No. 1 chromosome and related to daily weight gain of pigs and its application. Background technique [0002] "Du Dachang" Sanyuan hybrid pig is the most popular commercial pig hybrid strain in the world. Due to its fast growth, high lean meat rate and high feed conversion rate, Duroc pigs are often used as the terminal in breeding production. Paternal use. Average Daily Gain (ADG) refers to the average daily weight gain of pigs within 30-100 kg body weight, and is one of the indicators used to measure feed utilization efficiency. In addition, in the process of raising pigs, the cost of feed accounts for about 70% of the total cost of raising pigs. Therefore, improving the average daily gain of Duroc breeding pigs is an important breeding goal of breeding enterprises. The average daily gain is a trait ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 吴珍芳杨杰徐此能庄站伟郑恩琴蔡更元徐铮
Owner SOUTH CHINA AGRI UNIV
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