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SNP markers associated with dwarf traits in Chinese horses and their applications

A Chinese domestic and dwarf technology, applied in the field of genetic biology, can solve the problems of lack of breeding markers, low degree of selection and low degree of development and utilization, and achieve the effect of promoting the breeding process, simple operation, accurate and reliable method

Active Publication Date: 2021-02-02
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low level of domestic pony breeding, the lack of breeding markers, and the low degree of development and utilization, the resource advantages of pony horses in my country cannot be transformed into economic advantages.
At present, foreign countries have located HMGA2 and other gene loci that affect the body size traits of ponies through genome-wide scanning. However, previous studies have also shown that domestic and foreign ponies have their own independent formation mechanisms. Therefore, it is necessary to develop specific molecular markers for domestic ponies , applied to domestic pony breeding

Method used

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  • SNP markers associated with dwarf traits in Chinese horses and their applications
  • SNP markers associated with dwarf traits in Chinese horses and their applications
  • SNP markers associated with dwarf traits in Chinese horses and their applications

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Identification of polymorphic sites of TBX3 gene in Chinese national horse in embodiment 1

[0045] 1. Extract the genomic DNA from the blood of Chinese horses to be tested

[0046] Collected 210 ponies from Southwest China (including 19 from Debao, 34 from Baise, 35 from Wenshan, 20 from Yunnan, 15 from Lichuan, 32 from Jinjiang, 30 from Jianchang, 25 from Guizhou) and 308 normal-height horses (including 145 from Northwest China, 128 from Inner Mongolia, 24 from Northeast China, and 11 thoroughbreds), and 11 Przewalski's horses. A total of 529 individuals in 21 groups. Genomic DNA in blood was extracted by conventional methods.

[0047] Table 1 Sample Collection Form

[0048]

[0049]

[0050] 2. Amplify the nucleotide fragment containing the SNP site

[0051] Primers were designed according to the sequence of the TBX3 gene locus (gi 194246389:18100500-18101500 Equuscaballus isolate Twilight breed thoroughbred chromosome 8, EquCab2.0, wholegenome shotgun sequen...

Embodiment 2

[0059] Example 2 Correlation analysis and detection application of different genotypes and heights of Chinese national horses

[0060] The present invention has carried out a large number of studies on the correlation between genotype and height phenotype of Chinese national horses from different regions and breeds, and found that the SNP site provided by the present invention (located at the 501st position of the sequence shown in SEQ ID NO.1 , the polymorphism is G / A, corresponding to base pair 18205998 of horse chromosome 8) is significantly associated with domestic horse height (chi-square test P value<2.2e-16). The frequency of allele A in ponies was as high as 64.07%, while the frequency of allele G in common horses was 82.14%. The frequency of genotype AA was 65.95% in pony horses and 17.85% in common horses, and the average height of the horses with genotype AA was significantly shorter than that of horses with genotype GG (chi-square test P value<2.2e-16) , which is ...

Embodiment 3

[0062] Expanded population analysis of polymorphic sites in the TBX3 gene locus of 529 Chinese national horses. Using the chi-square test of R software to analyze, the above SNP site (located at the 501st position of the sequence shown in SEQ ID NO.1, the polymorphism is G / A, corresponding to the 18205998 base pair of horse chromosome 8) The frequency of allele A in ponies was significantly higher than that in other average-height horses (non-ponies) (P<2.2e-16). The correlation between the allele A of the SNP locus and the dwarf traits of Chinese horses was further verified (as shown in Table 2).

[0063] Table 2 The number of genotypes of SNP loci in Chinese pony and common horse breeds

[0064]

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Abstract

The invention relates to a SNP molecular marker related to the dwarf traits of Chinese horses. The SNP molecular marker is located at the 501st position of the sequence shown in SEQ ID NO.1, and the polymorphism is G / A, corresponding to the No. 8 horse chromosome 18205998 bp. The SNP markers related to the dwarf traits of Chinese horses and their applications of the present invention have the following advantages: (1) The molecular markers are not restricted by the age and sex of Chinese horses, and can be used for early breeding of Chinese horses, even just Accurate screening can be carried out at birth, which can significantly promote the breeding process of the dominant pony breeds of Chinese domestic horses; (2) the method for detecting the single nucleotide polymorphism of the Chinese domestic horse TBX3 gene is accurate, reliable, and easy to operate; (3) The detection of the SNP locus of the TBX3 gene of Chinese horses provides a scientific basis for the marker-assisted selection of the height and size of Chinese horses and the breeding of horses.

Description

technical field [0001] The invention belongs to the field of genetic biology, and specifically relates to a SNP marker related to the dwarf trait of Chinese horses and its application. Background technique [0002] SNP single nucleotide polymorphism site mainly refers to the polymorphism of DNA fragments caused by the variation of a single deoxynucleotide on the genome deoxynucleic acid (DNA) sequence. The polymorphism of SNP only involves the variation of a single base, and the expression forms include substitution, insertion and deletion. [0003] SNP detection methods currently commonly used include Sanger sequencing, DNA chips, time-of-flight mass spectrometry, and the latest high-throughput next-generation sequencing technologies. As a genetic marker, SNP has been widely used in research fields such as gene mapping, cloning, genetic breeding and genetic diversity. [0004] Transcription factor TBX3 (T-Box transcription factor 3) is one of the key genes regulating grow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124C12Q1/6876C12Q1/6837C12Q1/6895C12Q2600/13C12Q2600/16
Inventor 马月辉蒋琳刘雪雪浦亚斌张艳丽
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI