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A dyeing method and application of intermuscular spines of golden-threaded barb in Dianchi Lake

A dyeing method and technique of intermuscular spines, applied in the field of fish bone dyeing, can solve the problem of lack of a mature and effective method, and achieve the effects of avoiding the separation of intermuscular spines and muscles, shortening the clearing time, and fast dyeing

Active Publication Date: 2020-10-09
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no mature and effective method for staining the intermuscular spines of the golden-threaded barb in Dianchi Lake

Method used

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  • A dyeing method and application of intermuscular spines of golden-threaded barb in Dianchi Lake
  • A dyeing method and application of intermuscular spines of golden-threaded barb in Dianchi Lake

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The dyeing method of the intermuscular spines of the juvenile golden-threaded barb in Dianchi Lake that the present embodiment provides comprises the following steps:

[0040] 1. Sample fixation: The collected juveniles of Dianchichi barb were first anesthetized with MS-222 solution with a concentration of 0.01g / L for 4 minutes. After the fish were anesthetized, they were rinsed twice with deionized water for 2 minutes each time. ; Transfer to the plastic material tube that fills 2% paraformaldehyde solution subsequently and fix;

[0041] Conditions: fish body length at 25°C: anesthesia = 1cm: 10ml;

[0042] Preparation of anesthetic: Weigh 0.1 mg of MS-222 with a purity of 98% and dissolve it in 10 ml of deionized water;

[0043] Note: the anesthetic is prepared and used immediately, and the fixation time in 2% paraformaldehyde solution is 7 days.

[0044] 2. Removal of paraformaldehyde solution: wash the juvenile fish samples fixed by 2% paraformaldehyde solution wi...

Embodiment 2

[0066] The dyeing method of the intermuscular spines of the juvenile golden-threaded barb in Dianchi Lake that the present embodiment provides comprises the following steps:

[0067] 1. The samples were fixed at 23°C. The collected juvenile barb samples from Dianchi Lake were anesthetized with MS-222 solution with a concentration of 0.01g / L for 4 minutes, rinsed with deionized water twice, each time for 2 minutes, and then Transfer to and fix in the plastic material tube that fills 2% paraformaldehyde solution, wherein the preparation of anesthesia is to get MS-222 0.1mg of purity 98% to be dissolved in 10ml deionized water and form, consumption is according to fish body length: anesthesia =1cm:10ml is carried out, the anesthetic agent is prepared and used now, and the fixation time of 2% paraformaldehyde solution is 11 days;

[0068]2. In order to remove the excess paraformaldehyde solution of the sample, the fixed sample at 23°C was washed with deionized water for 3 times, e...

Embodiment 3

[0075] The dyeing method of the intermuscular spines of the juvenile golden-threaded barb in Dianchi Lake that the present embodiment provides comprises the following steps:

[0076] 1. The samples were fixed at 26°C. The collected juveniles of Dianchichi goldenrod were anesthetized sequentially with MS-222 solution with a concentration of 0.01g / L. The anesthesia time was 4 minutes, rinsed with deionized water twice, each After 2 minutes, transfer to a plastic material tube filled with 2% paraformaldehyde solution for fixation. The anesthetic is prepared by dissolving 0.1 mg of MS-222 with a purity of 98% in 10 ml of deionized water. Body length: anesthesia=1cm:10ml is carried out, and anesthesia is prepared and used now, and the fixed time of 2% paraformaldehyde solution is 15 days;

[0077] 2. In order to remove the excess paraformaldehyde solution of the sample, the fixed sample at 26°C was washed with deionized water for 3 times, each time for 10 minutes, and then deeply w...

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Abstract

The invention provides a dyeing method of sinocyclocheilus grahami intermuscular spines and an application thereof and belongs to the technical field of fish bone dyeing. The dyeing method comprises steps of (1) anesthetizing sinocyclocheilus grahami by utilizing anesthetic liquid, and then placing the sinocyclocheilus grahami in paraformaldehyde solution for immobilization; (2) cleaning the fixedsample, and placing the cleaned fixed sample into bleaching agent solution to remove body surface pigments so as to obtain a sample without the body surface pigments; 3) digesting the sample withoutthe body surface pigments in enzyme digestion solution till the muscle at a head of the sample is transparent to obtain a muscle transparent sample; 4) putting the muscle transparent sample into dyeing solution for intermuscular spines dyeing to obtain a sample which is entirely dyed; and (5) placing the sample which is wholly dyed into dehydration and decoloration solution for gradient dehydration and decoloration to obtain an intermuscular spine dyed sample. The method is advantaged in that the sinocyclocheilus grahami intermuscular spines can be rapidly dyed, and the requirement of clearlyobserving the expected effects such as the quantity, the form and the attachment position of the sinocyclocheilus grahami intermuscular spines can be met.

Description

technical field [0001] The invention belongs to the technical field of fish skeleton dyeing, and in particular relates to a method for dyeing intermuscular spines of the golden-threaded barb in Dianchi Lake and an application thereof. Background technique [0002] The golden-threaded barb Sinocyclocheilus grahami (Regan, 1904) in Dianchi Lake belongs to the genus Sinocyclocheilus (Cyprinidae) of Cypriniformes (Cypriniformes), and is commonly known as golden-threaded fish and small cave fish in the local area. This species is an atypical cave fish, mainly distributed in the rivers and Longtan of Dianchi Lake and its affiliated waters, and is endemic to Dianchi Lake. Because of its tender meat and delicious taste, it is listed as one of the four famous fish in Yunnan. At present, the researches on the golden-threaded barb in Dianchi Lake are mostly focused on broodstock breeding, fecundity, embryo survival rate, disease resistance, nutritional content of muscle, hybrid progen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 杨坤凤王晓爱张源伟潘晓赋杨君兴范伟
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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