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A method for rapid detection of pesticide residues in grains

A technology for pesticide residues and grains, which is applied in the field of rapid detection of pesticide residues in grains and rapid detection of methoxyacrylate fungicides in grains. It can solve the problems of inaccurate detection, errors, and small detection results, and improve extraction efficiency. , small environmental impact, efficient extraction effect

Active Publication Date: 2022-02-22
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to improper dispersion solvent and extraction agent, the detection results are small and large errors occur, which leads to inaccurate detection. Therefore, how to choose the extraction reagent of methoxyacrylate fungicide has become the key to accurately detect its content

Method used

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  • A method for rapid detection of pesticide residues in grains
  • A method for rapid detection of pesticide residues in grains
  • A method for rapid detection of pesticide residues in grains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for rapidly detecting methoxyacrylate fungicides in grains, using liquid chromatography for detection, external standard method for quantitative analysis, specifically as follows:

[0044] The methoxyacrylate fungicides referred to in the present invention include azoxystrobin, pyraclostrobin, and trifloxystrobin.

[0045] The present invention has many influencing factors on the pretreatment of grains, and its type and amount have a greater impact on the effect, and all need to be optimized. Therefore, the type and amount of extractant, the type and amount of dispersant, the amount of salt added, and the pH Factors such as value have carried out screening test, and purchased sample in the supermarket of applicant's place, detect same sample with prior art and the method of the present invention, to verify the reliability of the detection result of method of the present invention in practical application, test is as follows:

[0046] 1 Optimization of the type ...

Embodiment 2

[0082] The detection of methoxyacrylate fungicides in rice in embodiment 2

[0083] (1) Preparation of standard solution

[0084] Accurately weigh azoxystrobin, pyraclostrobin, and trifloxystrobin standard substances with a mass concentration of 97.0%, dissolve them in chromatographic methanol and prepare a mixed standard mother solution with a mass concentration of 2000mg / L, and use the gradient dilution method with chromatographic Dilute methanol to prepare standard working solutions of 20, 40, 200, 400 and 2000mg / L, the concentration in grains can reach 1, 2, 10, 20, 100mg / kg, and then put the standard series of solutions in a light-proof environment at 4°C save in .

[0085] (2) Sample pretreatment

[0086] Use a mill to crush the commercially available rice samples for later use; weigh 1 g of the sample in a centrifuge tube, add 50 μL of a mixed standard solution of azoxystrobin, pyraclostrobin, and trifloxystrobin in a series of gradients, add 2 mL of acetonitrile, and...

Embodiment 3

[0097] Embodiment 3: the detection of methoxyacrylate fungicides in wheat

[0098] (1) Preparation of standard solution

[0099] Accurately weigh azoxystrobin, pyraclostrobin, and trifloxystrobin standard substances with a mass concentration of 97.0%, dissolve them in chromatographic methanol and prepare a mixed standard mother solution with a mass concentration of 2000mg / L, and use the gradient dilution method with chromatographic Dilute methanol to prepare standard working solutions of 20, 40, 200, 400, 2000 mg / L, the concentration in grains can reach 1, 2, 10, 20, 100 mg / kg, and then put the standard series of solutions in a light-proof environment at 4°C save in .

[0100] (2) Sample pretreatment

[0101] Use a mill to crush the commercially available wheat samples for later use; weigh 1 g of the sample in a centrifuge tube, add 50 μL of a mixed standard solution of azoxystrobin, pyraclostrobin, and trifloxystrobin in a series of gradients, add 2 mL of acetonitrile, and ...

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Abstract

The invention relates to a method for quickly detecting pesticide residues in grains, and relates to a liquid chromatography detection method for methoxyacrylate fungicides. In this method, the grain sample is powdered, and the sample is weighed and added with a dispersant and sodium chloride. After mixing, the supernatant is taken out, and after filtering, the supernatant is mixed with the extractant and put into water to complete the dispersion. bath, the extractant is converted into a solid phase, transferred into a sample bottle, and detected by high-performance liquid chromatography, and quantitatively analyzed by an external standard method. The present invention can have the advantages of simplicity, high efficiency, and environmental protection, and can quickly measure pesticide residues in grain agricultural products, and the reproducibility, accuracy, precision and detection limit of the method can all meet the requirements of the pesticide residue analysis on grains Require.

Description

technical field [0001] The invention relates to a method for rapidly detecting pesticide residues in grains, in particular to a method for rapidly detecting methoxyacrylate fungicides in grains, and belongs to the field of grain detection. Background technique [0002] Cereals provide an enormous proportion of the world's population's dietary energy, protein and micronutrient needs, and unprocessed grains are low in fat and are good sources of fiber and phytochemicals. Nowadays, with people's attention to dietary health, the safety of grains and their processed products has also attracted much attention. The main problem in grains is that herbicides, fungicides, insecticides and other pesticides will not fully function after application, and some of them will remain in the grains. If you eat grains with pesticide residues, it will be very harmful to the human body. Methoxyacrylate fungicides are a new class of synthetic fungicides with a novel mode of action that interfere ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 王晓闻杜志毅荆旭陈振家伊莉付丽红朱俊玲
Owner SHANXI AGRI UNIV