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Preparation method of astragaloside

A technology of astragaloside IV and astragalus, which is applied in the field of preparation of astragaloside IV, can solve the problems of unstable product quality, large amount of acid, long time consumption, etc., and achieves a solution with high content of active ingredients, strong anti-oxidation, and avoidance of damage. Effect

Inactive Publication Date: 2019-12-10
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of acid used in the acid hydrolysis method, there are many by-products of acid degradation organic matter, resulting in a large amount of sewage discharge and serious environmental pollution, which limits the industrialization of astragaloglycoside IV.
Astragaloglucoside was extracted by pre-fermentation method, but the method was time-consuming and the product quality was not stable enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Prepare Astragalus membranaceus, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-to-liquid ratio of 1:5, add 17% cellulase by mass after uniformity, and enzymolyze at 55°C After 1h, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of enzyme-broken wall sediment, and add warm water (30°C), 50% absolute ethanol, 0.05mol / LPH value 3.7 at a material-to-liquid ratio of 1:5 citric acid-disodium hydrogen phosphate buffer solution, extract for 30min, and extract the material in a 30KHz ultrasonic device under the same conditions. , dried at 60°C to obtain astragaloside IV, and the content of astragaloside IV was 99.4% as detected by HPLC.

Embodiment 2

[0022] Prepare Astragalus membranaceus, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-to-liquid ratio of 1:5, add 17% cellulase by mass after uniformity, and enzymolyze at 55°C After 1h, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of enzyme-broken wall sediment, and add warm water (45°C), 55% absolute ethanol, 0.05mol / LPH value 3.7 at a material-to-liquid ratio of 1:5 citric acid-disodium hydrogen phosphate buffer solution, extracted for 35min, and the material was extracted in a 30KHz ultrasonic device under the same conditions. , dried at 60°C to obtain astragaloside IV, and the content of astragaloside IV was 99.6% as detected by HPLC.

Embodiment 3

[0024] Prepare Astragalus membranaceus, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-to-liquid ratio of 1:5, add 17% cellulase by mass after uniformity, and enzymolyze at 55°C After 1h, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of enzymatically broken wall sediment, and add warm water (50°C), 60% absolute ethanol, 0.05mol / LPH value 3.7 at a material-to-liquid ratio of 1:5 citric acid-disodium hydrogen phosphate buffer solution, extracted for 40min, and placed the material in a 30KHz ultrasonic device for extraction under the same conditions. After the extraction, centrifuged at 4000r / min for 10min, took the supernatant, and combined the two supernatants , dried at 60°C to obtain astragaloside IV, and the content of astragaloside IV was 99.8% as detected by HPLC.

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Abstract

The invention relates to a preparation method of astragaloside, in particular to a method for extracting astragaloside by adopting an enzyme method-ultrasonic wall breaking method. The method comprises the following processing steps: preparing astragalus membranaceus, pulverizing, grinding into powder, adding a citric acid-disodium hydrogen phosphate buffer solution with a pH value of 4.5 at a material-liquid ratio of 1 : 5; after uniformly mixing, adding 17% by mass of cellulase; carrying out enzymolysis at 55DEG C for 1h, and centrifugation for 10 min at 4000 r / min, collecting a supernate, taking a proper amount of an obtained enzymatic wall-breaking precipitate;, adding warm water at a solid-to-liquid ratio of 1: 5, 50 to 60% of absolute ethyl alcohol and 0.05 mol / L of citric acid-disodium hydrogen phosphate buffer solution at a pH value of 3.7; extracting for 30-40 minutes, carrying out ultrasonic-assisted extraction under the same conditions, centrifuging for 10 minutes at the speed of 4000 r / min after the extraction is finished, taking an obtained supernate, combining the two supernates obtained in the former two steps, and drying at the temperature of 60 DEG C to obtain astragaloside. The method has the advantages of high efficiency and high content of effective components in the solution.

Description

technical field [0001] The invention relates to a preparation method of astragaloside IV, which belongs to the field of extraction of effective components of traditional Chinese medicines. Background technique [0002] Astragalus is a pure natural product that people often eat. There is a popular saying among the people that "drink Astragalus soup often to prevent disease and maintain health", which means that Astragalus is often fried in soup or soaked in water instead of tea, which has a good effect on disease prevention and health care. Both astragalus and ginseng are good medicines for invigorating qi, and ginseng focuses on invigorating vital qi, restoring yang and reversing adversity, and is often used in emergencies such as collapse and shock with better results. Astragalus is mainly used to invigorate deficiency, and it is often used for those who have been weakened for a long time, have weak speech, and weak pulse. Some people are prone to catch a cold when the wea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J53/00
CPCC07J53/004
Inventor 杨成东
Owner NANJING ZELANG AGRI DEV
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